Why lamin-b1 expression in aging cell but not young cell ?

14 October 2023 1 8K Report

I have used miRNA-mimic transfect the HUVEC cell. and than RIPA lysis buffer to extract the cell protein. BUT the western blotting result is interesting. the mimic transfect cell express the lamin-B1, but the control group don't.

AS our previous study, the miRNA will induce the cell sencence. I don't know why.

primary antibody

lamin-B1 #365962

Luigi Marongiu

Could it be that cells with different ages express different levels of laminin-1? On this paper DOI: 10.1002/aja.1002030404, I have found that "Different temporal patterns of laminin alpha 1, beta 1, and gamma 1 subunit chain expression were observed" ...

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