I am facing differences in MSC proliferation after following the same isolation protocol. Pig, rabbit, and rat seem to proliferate well and stable (with minor differences) but mouse derived MSC grow very slow and show a fast change in morphology (cells flatten, show stress fibers). Can you tell me about species-dependent differences of MSC?
You can check the work of Donald Phinney (Scripps Research Institute, Florida), who first established culture conditions for mouse BMSC in vitro isolation after for a long time it was not possible to culture them in the same way as human or other species' ones.
Hello Mathias;
I will isolate MSCs from rat bone marrow. It will be my first animal study. Which markers do you use? If you could help me I would appreciate it.
Thanks
Berrin
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