Mathias Roth

14 Questions 17 Answers 0 Followers

Questions related from Mathias Roth

How to plot data, if in a pair of values (for a paired t-test / Wilcoxon test) one of the two values is missing?

I have a dataset with 100 paired values (pre- and post intervention X). In 20 couples 1 of the 2 values is missing. When I compare pre- to post-intervention values, those 2 couples / rows will be...

05 December 2020 3,153 9 View

Is the measured area in an image with a bigger scale in Y than in X dependent of the orientation?

I have an image with a bigger scale in Y than in X. In this image I want to measure several areas. Now I need to know, if the true area is dependent of the orientation of the object / area I...

07 June 2018 9,993 5 View

For sufficient Cre-ERT2 induction, what dosage and resorption time of subcutaneous tamoxifen pellets do you recommend?

In a in vivo mouse project tamoxifen will be administered in subcutaneous implanted pellets. What dosage and resorption time of subcutaneous tamoxifen pellets (e.g. 100 mg over 21 days = daily...

15 October 2015 7,604 1 View

What galenic formulation of tamoxifen (tm) should be used for the application of subcutaneous tamoxifen pellets in mice: tm free base or tm citrate?

In a in vivo mouse project tamoxifen will be administered in subcutaneous implanted pellets. Two forms of pellets are available: Tamoxifen free base and Tamoxifen citrate. What galenic formulation...

15 October 2015 800 1 View

What is the best method for probe retrieval, storage and transport in microbiological analysis of ocular infections?

In ocular infections, especially in infectious keratitis, microbiological analysis is difficult due to the small amount of material / probe. What is the best method: directly inoculate the swab or...

28 August 2014 3,694 1 View

What is a good paper on the effect of cell sorting on the sorted cells?

Can anyone recommend literature on the effect of cell sorting on proliferation capacity / stress response / senescence of the sorted cells (especially MSC)? How is the cell size influencing the...

03 August 2014 827 2 View

Which software is good to handle database setup from the beginning?

I need to set up a database with the following requirements: - easy handling (data entry, read out / statistics, changing the data entry interface and adding new parameters) - in best case it...

11 April 2014 8,829 6 View

What antibody is working properly?

I analyzed supposedly MSC by flow cytometry and immunofluorescent staining with 2 different CD73 antibodies. As MSC the cells are supposed to be positive for CD73. The immunostaining shows a nice...

06 February 2014 7,310 2 View

Is it possible to do a flow cytometry / facs analysis of cells DIRECTLY after they were solved out of glandular tissue?

I wonder if it is possible to dissociate glandular tissue by an agent like collagenase / accutase etc. and do an flow cytometry / cell sorting right afterwards with those cells. Or do you think...

31 July 2013 4,736 8 View

In osteogenic differentiation of murine MSC (Mesenchymal Stem Cells) in hypoxia I see spontaneous adipogenic differentiation as well. Does anyone else see this phenomena?

In osteogenic differentiation of murine MSC in hypoxia I see spontaneous adipogenic differentiation. In 21% O2 I do not have any problems. What might be the reason?

17 July 2013 2,467 7 View

What type of colony forming efficiency assay / CFU-F assay do you recommend for MSC?

There are several different methods described for CFU-F assays for MSC. E.g. in the book "Mesenchymal Stem Cell Assays and Applications" there are several partly very different methods described....

05 July 2013 2,030 4 View

After transfer of MSC (Mesenchymal Stem Cell) isolated by explant-culture, almost no cells stay alive. What might be the reason?

We are trying to isolate MSC by explant cultures of tissue pieces. In a 10 cm2 petridish (polystyrene) there is a good number of cells growing out of the explants. After trypsination (warmed up...

27 March 2013 5,660 9 View

Without stating the minimal criteria (CD73, CD90 and CD105), how can you be sure you are talking about MSC?

In an article I just read, they are talking about the isolation of MSC from the lacrimal gland: You, S., Isolation and propagation of mesenchymal stem cells from the lacrimal gland. PMID:...

31 January 2013 1,646 10 View

Does the topical application of mesenchymal stem cells (MSC) make sense in mucous membrane pemphigoid?

Dear colleagues, we know about the anti-inflammatory and anti-fibrotic properties of MSC, as reviewed e.g. in this article: https://www.nature.com/articles/s41401-022-00952-0 Mucous membrane...

01 January 1970 8,351 0 View