14 Questions 17 Answers 0 Followers
Questions related from Mathias Roth
I have a dataset with 100 paired values (pre- and post intervention X). In 20 couples 1 of the 2 values is missing. When I compare pre- to post-intervention values, those 2 couples / rows will be...
05 December 2020 3,155 9 View
I have an image with a bigger scale in Y than in X. In this image I want to measure several areas. Now I need to know, if the true area is dependent of the orientation of the object / area I...
07 June 2018 9,993 5 View
In a in vivo mouse project tamoxifen will be administered in subcutaneous implanted pellets. What dosage and resorption time of subcutaneous tamoxifen pellets (e.g. 100 mg over 21 days = daily...
15 October 2015 7,605 1 View
In a in vivo mouse project tamoxifen will be administered in subcutaneous implanted pellets. Two forms of pellets are available: Tamoxifen free base and Tamoxifen citrate. What galenic formulation...
15 October 2015 800 1 View
In ocular infections, especially in infectious keratitis, microbiological analysis is difficult due to the small amount of material / probe. What is the best method: directly inoculate the swab or...
28 August 2014 3,699 1 View
Can anyone recommend literature on the effect of cell sorting on proliferation capacity / stress response / senescence of the sorted cells (especially MSC)? How is the cell size influencing the...
03 August 2014 829 2 View
I need to set up a database with the following requirements: - easy handling (data entry, read out / statistics, changing the data entry interface and adding new parameters) - in best case it...
11 April 2014 8,830 6 View
I analyzed supposedly MSC by flow cytometry and immunofluorescent staining with 2 different CD73 antibodies. As MSC the cells are supposed to be positive for CD73. The immunostaining shows a nice...
06 February 2014 7,310 2 View
I wonder if it is possible to dissociate glandular tissue by an agent like collagenase / accutase etc. and do an flow cytometry / cell sorting right afterwards with those cells. Or do you think...
31 July 2013 4,737 8 View
In osteogenic differentiation of murine MSC in hypoxia I see spontaneous adipogenic differentiation. In 21% O2 I do not have any problems. What might be the reason?
17 July 2013 2,468 7 View
There are several different methods described for CFU-F assays for MSC. E.g. in the book "Mesenchymal Stem Cell Assays and Applications" there are several partly very different methods described....
05 July 2013 2,031 4 View
We are trying to isolate MSC by explant cultures of tissue pieces. In a 10 cm2 petridish (polystyrene) there is a good number of cells growing out of the explants. After trypsination (warmed up...
27 March 2013 5,661 9 View
In an article I just read, they are talking about the isolation of MSC from the lacrimal gland: You, S., Isolation and propagation of mesenchymal stem cells from the lacrimal gland. PMID:...
31 January 2013 1,646 10 View
Dear colleagues, we know about the anti-inflammatory and anti-fibrotic properties of MSC, as reviewed e.g. in this article: https://www.nature.com/articles/s41401-022-00952-0 Mucous membrane...
01 January 1970 8,351 0 View