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Questions related from Mathias Roth
I have a dataset with 100 paired values (pre- and post intervention X). In 20 couples 1 of the 2 values is missing. When I compare pre- to post-intervention values, those 2 couples / rows will be...
05 December 2020 3,216 9 View
I have an image with a bigger scale in Y than in X. In this image I want to measure several areas. Now I need to know, if the true area is dependent of the orientation of the object / area I...
07 June 2018 10,064 5 View
I remember to have read about the side effects of ablation of Nestin in adolescent mice - not in embryos (e.g. in a model with Nestin/Cre-ERT2/diphtheria toxin model): tremor, shivering and ataxia...
30 March 2016 2,184 0 View
In a in vivo mouse project tamoxifen will be administered in subcutaneous implanted pellets. What dosage and resorption time of subcutaneous tamoxifen pellets (e.g. 100 mg over 21 days = daily...
15 October 2015 7,642 1 View
In a in vivo mouse project tamoxifen will be administered in subcutaneous implanted pellets. Two forms of pellets are available: Tamoxifen free base and Tamoxifen citrate. What galenic formulation...
15 October 2015 849 1 View
In ocular infections, especially in infectious keratitis, microbiological analysis is difficult due to the small amount of material / probe. What is the best method: directly inoculate the swab or...
28 August 2014 3,757 1 View
Can anyone recommend literature on the effect of cell sorting on proliferation capacity / stress response / senescence of the sorted cells (especially MSC)? How is the cell size influencing the...
03 August 2014 881 2 View
I need to set up a database with the following requirements: - easy handling (data entry, read out / statistics, changing the data entry interface and adding new parameters) - in best case it...
11 April 2014 8,878 6 View
I analyzed supposedly MSC by flow cytometry and immunofluorescent staining with 2 different CD73 antibodies. As MSC the cells are supposed to be positive for CD73. The immunostaining shows a nice...
06 February 2014 7,362 2 View
I wonder if it is possible to dissociate glandular tissue by an agent like collagenase / accutase etc. and do an flow cytometry / cell sorting right afterwards with those cells. Or do you think...
31 July 2013 4,785 8 View
In osteogenic differentiation of murine MSC in hypoxia I see spontaneous adipogenic differentiation. In 21% O2 I do not have any problems. What might be the reason?
17 July 2013 2,513 7 View
There are several different methods described for CFU-F assays for MSC. E.g. in the book "Mesenchymal Stem Cell Assays and Applications" there are several partly very different methods described....
05 July 2013 2,082 4 View
I am facing differences in MSC proliferation after following the same isolation protocol. Pig, rabbit, and rat seem to proliferate well and stable (with minor differences) but mouse derived MSC...
18 May 2013 5,608 3 View
We are trying to isolate MSC by explant cultures of tissue pieces. In a 10 cm2 petridish (polystyrene) there is a good number of cells growing out of the explants. After trypsination (warmed up...
27 March 2013 5,707 9 View
As we are facing difficulties with transfer of MSC to chamberslides right now we dont have a usable method for staining of cultured MSC with the conventional fluorescence microscope. Do you have...
27 March 2013 3,436 3 View
In an article I just read, they are talking about the isolation of MSC from the lacrimal gland: You, S., Isolation and propagation of mesenchymal stem cells from the lacrimal gland. PMID:...
31 January 2013 1,684 10 View
Dear colleagues, we know about the anti-inflammatory and anti-fibrotic properties of MSC, as reviewed e.g. in this article: https://www.nature.com/articles/s41401-022-00952-0 Mucous membrane...
01 January 1970 8,403 0 View
