Which dye I should use for apoptosis assay with Caco-2 cells?

I want to perform apoptosis assay of caco-2 cells. I want to measure the live and dead cells. I don't have access to Flow cytometry, so I would like to use stain and then count the cell number...

08 September 2018 1,353 3 View

How can i separate highly polar compounds?

I have separated phytochemicals from water fraction using reverse phase silica and then ran through reverse phase TLC plates using water and methanol as solvent. But I can't separate them. Anybody...

07 August 2018 2,377 10 View

I would like to perform HPLC/MS of a plant extract. Can anyone suggest me with the method of doing it?

I have hexane fraction of a plant leaf. I have sub-fractioned it by running on normal phase silica column. Now I want to run those sub-fractions in analytical HPLC/MS with PDA detector. Can any...

07 August 2018 6,047 6 View

Which one is better for treating experimental colitis, sulfasalazine or 5-amino salicylic acid?

I want to induce colitis by DSS and then use phytochemical to check it's effect in disease amelioration. In this process i want to use standard drug to check the performance of my phytochemical.

04 May 2018 2,013 2 View

Which mice is better for DSS induced experimental colitis, BALB/c or C57BL/6?

I want to induce experimental colitis by oral administration of 5% DSS. Now as I see there are many papers suggesting different things about experimental mice for DSS induce colitis model, I am...

04 May 2018 5,248 5 View

Membrane used in Western blotting

Last week I performed Western blotting and I tried to separate a tight junction protein, zonulin, using Nitrocellulose membrane. But after performing the experiment I read that for separating low...

01 January 1970 5,440 4 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View