Which one is better for treating experimental colitis, sulfasalazine or 5-amino salicylic acid?

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Which dye I should use for apoptosis assay with Caco-2 cells?

I want to perform apoptosis assay of caco-2 cells. I want to measure the live and dead cells. I don't have access to Flow cytometry, so I would like to use stain and then count the cell number...

08 September 2018 1,378 3 View

Why is there no change in alkaline phosphatase in my caco-2 cell culture?

Caco-2 cells were seeded in transwell (Polyester Membrane, Growth Surface Area 1.12 cm2, 0.4 mm pore size, Corning, Cat. No. 3460). The transepithelial electrical resistance (TEER) was measured...

08 September 2018 799 0 View

How can i separate highly polar compounds?

I have separated phytochemicals from water fraction using reverse phase silica and then ran through reverse phase TLC plates using water and methanol as solvent. But I can't separate them. Anybody...

07 August 2018 2,411 10 View

I would like to perform HPLC/MS of a plant extract. Can anyone suggest me with the method of doing it?

I have hexane fraction of a plant leaf. I have sub-fractioned it by running on normal phase silica column. Now I want to run those sub-fractions in analytical HPLC/MS with PDA detector. Can any...

07 August 2018 6,088 6 View

Which mice is better for DSS induced experimental colitis, BALB/c or C57BL/6?

I want to induce experimental colitis by oral administration of 5% DSS. Now as I see there are many papers suggesting different things about experimental mice for DSS induce colitis model, I am...

04 May 2018 5,273 5 View

Membrane used in Western blotting

Last week I performed Western blotting and I tried to separate a tight junction protein, zonulin, using Nitrocellulose membrane. But after performing the experiment I read that for separating low...

01 January 1970 5,465 4 View

Is changing the medium during the experiment overestimate the results?

I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...

07 August 2024 2,283 2 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

The aqueous fraction of the hydroethanolic extract is showing the presence of palmitic acid. What is the mechanism responsible ?

Palmitic acid presence in aqueous fraction

05 August 2024 8,624 4 View

Which solvent is better to dissolve with secondary metabolites extracted from fungi?

I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...

03 August 2024 4,725 2 View

Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...

03 August 2024 2,730 1 View

How to convert g/kg Humic acid dose to kg/ha?

I used humic acid at 0.044 g/kg soil in my pot experiment. But finally, I have to recommend kg/ha. Each pot's soil weight was 11 kg. What is the solution?

02 August 2024 7,186 6 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

How to maintain the drug concentration in cell culture study for long term?

I am working in the filed of long acting drugs with Nano formulation. I want to study the efficacy of my formulation in 2D cell cultures while this required media change on every two days. In such...

24 July 2024 8,696 0 View

Why is trifluoroacetic acid (TFA) used in c-18 column?

I would like to know the role of the TFA (trifluoroacetic acid) used on reverse phase chromatography c-18 column. It's is often used with a concentration of 0.1% on the mobile phase.

23 July 2024 8,075 3 View

Can we add calcium carbonate to any media as an indicator for lactic acid producing bacteria?

As mentioned in literature the addition of CaCO3 to MRS Agar media is considered as an indicator for lactic acid producing bacteria, so I wonder if we can add CaCO3 calcium carbonate to any other...

20 July 2024 7,504 0 View