Which one is better for treating experimental colitis, sulfasalazine or 5-amino salicylic acid?

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Which dye I should use for apoptosis assay with Caco-2 cells?

I want to perform apoptosis assay of caco-2 cells. I want to measure the live and dead cells. I don't have access to Flow cytometry, so I would like to use stain and then count the cell number...

08 September 2018 1,353 3 View

Why is there no change in alkaline phosphatase in my caco-2 cell culture?

Caco-2 cells were seeded in transwell (Polyester Membrane, Growth Surface Area 1.12 cm2, 0.4 mm pore size, Corning, Cat. No. 3460). The transepithelial electrical resistance (TEER) was measured...

08 September 2018 779 0 View

How can i separate highly polar compounds?

I have separated phytochemicals from water fraction using reverse phase silica and then ran through reverse phase TLC plates using water and methanol as solvent. But I can't separate them. Anybody...

07 August 2018 2,377 10 View

I would like to perform HPLC/MS of a plant extract. Can anyone suggest me with the method of doing it?

I have hexane fraction of a plant leaf. I have sub-fractioned it by running on normal phase silica column. Now I want to run those sub-fractions in analytical HPLC/MS with PDA detector. Can any...

07 August 2018 6,047 6 View

Which mice is better for DSS induced experimental colitis, BALB/c or C57BL/6?

I want to induce experimental colitis by oral administration of 5% DSS. Now as I see there are many papers suggesting different things about experimental mice for DSS induce colitis model, I am...

04 May 2018 5,248 5 View

Membrane used in Western blotting

Last week I performed Western blotting and I tried to separate a tight junction protein, zonulin, using Nitrocellulose membrane. But after performing the experiment I read that for separating low...

01 January 1970 5,440 4 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

How do I choose contrasts in SPM8?

I am learning how to use SPM for PET and PET-MR analysis, but I haven't found any great resources that explain how contrasts work. I have a series of PET images where our hypothesis argues that...

24 February 2021 1,534 2 View

Can I use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography?

Hi! I would want to know if I can use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography. This column has a polyethylene glycol phase, but I can't find the manual to check...

22 February 2021 730 2 View

Can you perform the Murexide test on samples fixed in Formalin?

I have a fixed sample and would like to use the murexide reaction to test for uric acid composition. I no longer have fresh samples

22 February 2021 1,328 1 View

Is there an ideal incubation time of RGO-modified SPCE before subsequent use, i.e., electrochemical characterization or further modification?

I notice that upon washing and air-drying of my RGO/SPCE surface and characterizing again via DPV, the current response towards ferricyanide probe significantly decreases. My worry is that the...

22 February 2021 5,324 3 View

Does anyone have any ideas why drug-resistant malaria is increasing in Southeast Asia even when those drugs are no longer used?

When dealing with bacterial toxins, there are three varieties of microbes that develop in your petri dish: toxic, resistant, and susceptible. Initially, there are only toxic and vulnerable...

21 February 2021 6,084 5 View