Imam Hossen

7 Questions 14 Answers 0 Followers

Questions related from Imam Hossen

Why is there no change in alkaline phosphatase in my caco-2 cell culture?

Caco-2 cells were seeded in transwell (Polyester Membrane, Growth Surface Area 1.12 cm2, 0.4 mm pore size, Corning, Cat. No. 3460). The transepithelial electrical resistance (TEER) was measured...

09 September 2018 788 0 View

Which dye I should use for apoptosis assay with Caco-2 cells?

I want to perform apoptosis assay of caco-2 cells. I want to measure the live and dead cells. I don't have access to Flow cytometry, so I would like to use stain and then count the cell number...

09 September 2018 1,359 3 View

How can i separate highly polar compounds?

I have separated phytochemicals from water fraction using reverse phase silica and then ran through reverse phase TLC plates using water and methanol as solvent. But I can't separate them. Anybody...

08 August 2018 2,385 10 View

I would like to perform HPLC/MS of a plant extract. Can anyone suggest me with the method of doing it?

I have hexane fraction of a plant leaf. I have sub-fractioned it by running on normal phase silica column. Now I want to run those sub-fractions in analytical HPLC/MS with PDA detector. Can any...

08 August 2018 6,056 6 View

Which mice is better for DSS induced experimental colitis, BALB/c or C57BL/6?

I want to induce experimental colitis by oral administration of 5% DSS. Now as I see there are many papers suggesting different things about experimental mice for DSS induce colitis model, I am...

05 May 2018 5,257 5 View

Which one is better for treating experimental colitis, sulfasalazine or 5-amino salicylic acid?

I want to induce colitis by DSS and then use phytochemical to check it's effect in disease amelioration. In this process i want to use standard drug to check the performance of my phytochemical.

05 May 2018 2,023 2 View

Membrane used in Western blotting

Last week I performed Western blotting and I tried to separate a tight junction protein, zonulin, using Nitrocellulose membrane. But after performing the experiment I read that for separating low...

01 January 1970 5,448 4 View