What are the possible reasons for a primer dimer to occur in a PCR, LAMP reaction?

I am currently working in an isothermal amplification technique called PSR ( Polymerase spiral Reaction). I have followed the reaction mixture from previous articles but i am getting primer dimers...

15 February 2020 2,826 3 View

Issue with PCR amplification of an amplicon ?

I have done a 16S rDNA amplification of a metagenomic sample. I got a clear band near 1.5kb in the gel. Later on purification and again when we do the same PCR with the exact same primers and...

25 February 2019 4,532 5 View

Can any one give the optimum conditions to prepare Cdo-Zno thin film by spin coting technique?

can any one give the optimum conditions to prepare Cdo-Zno thin film by spin coating technique?

19 February 2019 1,979 2 View

Can TITANIUM butoxide monomer chemicals are useful to prepare titania nanoparticles?

is titania nanoparticle is prepared from titanium butoxide monomer?

29 January 2018 9,567 3 View

How to prevent DNA binding protein precipitation while concentating?

I have DNA binding protein and I am getting a conc, of 50um from 50ml BL21 DE3 culture (I will be scaling this up to 1mM). The protein is Hexa-His tagged at C-terminus and to elute the protein...

23 August 2017 9,167 3 View

Can anyone help me in doing the WLF fitting of rheology data for thermosets?

I am working on thermoset systems and recently I finished the rheology frequency sweep experiments I need to do the WLF fitting.

01 March 2014 9,606 2 View

How can i isolate DspE protein ?

I want to isolate DspE from Erwinia amylovora. Please suggest me the procedure which required to isolate the DspE Protein. I don't want any cloning experiments.

30 July 2013 9,971 2 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

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02 March 2021 3,060 3 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

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28 February 2021 9,936 2 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View