How does CRISPR-Cas system in bacteria recognize bacteriophage or viral DNA as lethal target?

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Hanna Alalam

Well it does mediate resistance to conjugative elements and plasmids. Please refer to the link below:

Article CRISPR-Cas systems preferentially target the leading regions...

Rittwika Bhattacharya

Probably having to do with chi sites. Chi elements are unique for bacteria and for bacteriophages.

https://en.wikipedia.org/wiki/Chi_site

I would like to know the detailed protocol, especially about Cas9-gRNA preparation.