How does CRISPR-Cas system in bacteria recognize bacteriophage or viral DNA as lethal target?

More Sarath Reghunathan's questions See All

What are the possible reasons for a primer dimer to occur in a PCR, LAMP reaction?

I am currently working in an isothermal amplification technique called PSR ( Polymerase spiral Reaction). I have followed the reaction mixture from previous articles but i am getting primer dimers...

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Issue with PCR amplification of an amplicon ?

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Can any one give the optimum conditions to prepare Cdo-Zno thin film by spin coting technique?

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Why is the fungal culture not growing in the agar medium but grows in broth ?

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23 December 2018 2,632 6 View

Can TITANIUM butoxide monomer chemicals are useful to prepare titania nanoparticles?

is titania nanoparticle is prepared from titanium butoxide monomer?

29 January 2018 9,567 3 View

How to prevent DNA binding protein precipitation while concentating?

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Can anyone help me in doing the WLF fitting of rheology data for thermosets?

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How can i isolate DspE protein ?

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Can we classify HV and LV lines based on line length?

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Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

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Is there a classification scheme for rock cavities or caverns with respect to their size and shape?

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02 March 2021 4,511 3 View

Is there a powerful system for the security of the systems distributed on IoT systems?

02 March 2021 3,858 10 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

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Which is suitable for use with Python? MySQL or SQL Server?

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Which cell line should we use to perform biological dosimetry experiments?

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How does an antibody have full intact antibody + half antibody?

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28 February 2021 1,577 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

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Hanna Alalam

Well it does mediate resistance to conjugative elements and plasmids. Please refer to the link below:

Article CRISPR-Cas systems preferentially target the leading regions...

Rittwika Bhattacharya

Probably having to do with chi sites. Chi elements are unique for bacteria and for bacteriophages.

https://en.wikipedia.org/wiki/Chi_site

I would like to know the detailed protocol, especially about Cas9-gRNA preparation.