Sarath Reghunathan

5 Questions 14 Answers 0 Followers

Questions related from Sarath Reghunathan

What are the possible reasons for a primer dimer to occur in a PCR, LAMP reaction?

I am currently working in an isothermal amplification technique called PSR ( Polymerase spiral Reaction). I have followed the reaction mixture from previous articles but i am getting primer dimers...

16 February 2020 2,915 3 View

How to overcome contamination in a fungal plate ? and how to purify fungi from a contaminated plate?

hello all, i am currently facing a big trouble with contamination in my culture plate. i am currently working with Trichophyton rubrum, Microsporum canis. i usually do well diffusion assay to...

05 March 2019 9,157 0 View

Issue with PCR amplification of an amplicon ?

I have done a 16S rDNA amplification of a metagenomic sample. I got a clear band near 1.5kb in the gel. Later on purification and again when we do the same PCR with the exact same primers and...

26 February 2019 4,599 5 View

Is it like a balance, each bacteria has a nemesis, a bacteriophage that is capable of killing it ?

Just had a thought !! is it possible that all the bacteria in the world have a bacteriophage waiting to kill them up ? like, one bacteria, one bacteriophage ? does it have any evolutionary...

10 January 2019 6,651 4 View

Why is the fungal culture not growing in the agar medium but grows in broth ?

Trichophyton rubrum and Microsporum canis are the 2 fungal cultures that i need to assay ( well diffusion assay), but i have encountered a problem, the fungal culture is not growing in the plate....

24 December 2018 2,702 6 View