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Questions related from Sarath Reghunathan
I am currently working in an isothermal amplification technique called PSR ( Polymerase spiral Reaction). I have followed the reaction mixture from previous articles but i am getting primer dimers...
16 February 2020 2,932 3 View
i would like to know, how viral DNA's are unique when compared to that of the bacterial DNA. Why doesn't the CRISPR-Cas system activate for a bacterial DNA when it gets transferred to the...
20 June 2019 7,919 3 View
hello all, i am currently facing a big trouble with contamination in my culture plate. i am currently working with Trichophyton rubrum, Microsporum canis. i usually do well diffusion assay to...
05 March 2019 9,168 0 View
I have done a 16S rDNA amplification of a metagenomic sample. I got a clear band near 1.5kb in the gel. Later on purification and again when we do the same PCR with the exact same primers and...
26 February 2019 4,618 5 View
i have a small trouble in collecting the spores from fungal plates. it would be of great help if anyone could help me with a suggestion and a reference paper regarding this. i have tried pouring...
06 February 2019 4,190 5 View
Just had a thought !! is it possible that all the bacteria in the world have a bacteriophage waiting to kill them up ? like, one bacteria, one bacteriophage ? does it have any evolutionary...
10 January 2019 6,659 4 View
Trichophyton rubrum and Microsporum canis are the 2 fungal cultures that i need to assay ( well diffusion assay), but i have encountered a problem, the fungal culture is not growing in the plate....
24 December 2018 2,718 6 View
