Sarath Reghunathan

9 Questions 21 Answers 0 Followers

Questions related from Sarath Reghunathan

What are the possible reasons for a primer dimer to occur in a PCR, LAMP reaction?

I am currently working in an isothermal amplification technique called PSR ( Polymerase spiral Reaction). I have followed the reaction mixture from previous articles but i am getting primer dimers...

16 February 2020 2,987 3 View

How does CRISPR-Cas system in bacteria recognize bacteriophage or viral DNA as lethal target?

i would like to know, how viral DNA's are unique when compared to that of the bacterial DNA. Why doesn't the CRISPR-Cas system activate for a bacterial DNA when it gets transferred to the...

20 June 2019 7,967 3 View

How to overcome contamination in a fungal plate ? and how to purify fungi from a contaminated plate?

hello all, i am currently facing a big trouble with contamination in my culture plate. i am currently working with Trichophyton rubrum, Microsporum canis. i usually do well diffusion assay to...

05 March 2019 9,241 0 View

Issue with PCR amplification of an amplicon ?

I have done a 16S rDNA amplification of a metagenomic sample. I got a clear band near 1.5kb in the gel. Later on purification and again when we do the same PCR with the exact same primers and...

26 February 2019 4,683 5 View

How do i collect spores from Trichophyton rubrum, M. canis and Epidermophyton plate for MIC and other quantitative antimicrobial assays ?

i have a small trouble in collecting the spores from fungal plates. it would be of great help if anyone could help me with a suggestion and a reference paper regarding this. i have tried pouring...

06 February 2019 4,233 5 View

What does the superscripted alphabet in the data table represent in some research articles ?

recently i encountered some research papers where they have super-scripted some alphabets on top of the values. can somebody please explain me what they are trying to infer. i have attached the...

06 February 2019 5,689 0 View

Will the molecular weight of the Nanoparticle change when it is being doped with another element ?

i need to know whether the molecular weight of a nanoparticle change if it is being doped with another element. if it changes how does one calculate molarity using the pure and doped. what changes...

24 January 2019 7,518 0 View

Is it like a balance, each bacteria has a nemesis, a bacteriophage that is capable of killing it ?

Just had a thought !! is it possible that all the bacteria in the world have a bacteriophage waiting to kill them up ? like, one bacteria, one bacteriophage ? does it have any evolutionary...

10 January 2019 6,707 4 View

Why is the fungal culture not growing in the agar medium but grows in broth ?

Trichophyton rubrum and Microsporum canis are the 2 fungal cultures that i need to assay ( well diffusion assay), but i have encountered a problem, the fungal culture is not growing in the plate....

24 December 2018 2,799 6 View