What is the maximum voltage a piezoelectric PZT layer can handle?

I have a circular PZT layer deposited by PLD method. The dimensions are: 20um diameter and 350nm thick. It has 100nm Pt electrodes sputtered on the top and bottom. I would like to know what is the...

25 January 2021 688 5 View

How to define hyper elastic UMAT in a UEL?

Hi all, I am working on Phase-Field UEL for hyperelastic materials. I am using codes of Dr Molnar ( http://www.molnar-research.com/tutorials_videos_3.html) to develop hyperelastic material. I...

07 April 2020 4,243 1 View

Why some materials getting overpotential while doing potentiodynamic test by using iviumstat chemical analyzer (especially for aluminium alloys)?

I am working with aluminium - chromium alloys. I have a solid samples of this alloy after prepared by powder Metallurgy route. I am facing a difficulty when doing a potentiodynamic polarization...

12 September 2018 5,649 3 View

How to remove Edge effect in continuous wavelet transform using MATLAB tool box?

It is not clear to me how the CWT function handles edge effects for a finite length time series. How to remove this edge effect in wavelets using the MATLAB toolbox and any procedure is there for it?

15 April 2018 7,453 5 View

How to remove phage DNA from plasmid.

can anyone plese let me know how to clean phage contaminated plasmid.

11 April 2017 3,815 4 View

How to decompose vibration signal to get the damping ratios using wavelet analysis? Which wavelet is most suitable for analyzing vibration signal?

18 May 2016 3,224 2 View

Can we synthesize Pd (111) in any method?

In some literature they have mentioned about the performance of the Pt or Pd based on its structure.

11 December 2013 8,854 6 View

How can I extract BPA from treated urine sample?

I have urine samples collected from treated animals, which i want to analyse on HPLC. How can I extract the BPA?

09 November 2012 4,305 5 View

Can any one explain what is the correct mechanism of hair growth?

Trigger factors for hair growth at cellular and molecular level??

01 January 1970 4,171 2 View

What are the properties required to manufacture a boring bar? And also tell me the criteria for selecting a boring bar material?

I want to manufacture a boring bar with composite material for that i require the following: 1. What properties a boring bar should have 2. What material i can use to manufacture a boring bar, 3....

01 January 1970 4,322 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View