I am using P.chrysogenum for my experiment. After incubation period spores grew without any contamination. I stored it in a refrigerator. After 10 days the culture medium turned into black. There is no visible contamination on top view of culture/ spore.
Pannerselvam Saravanan
Mature fungal mycelium produce black pigment. This pigment diffused into the medium. So the media colour changed to black.
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Ismail Abdel-Rahim
I didn't see this phenomenon. Penicillium chrysogenum usually produces yellow colour in the cultural medium, thus identification of the fungus need some confirmation.
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