Hello everyone. I need some help troubleshooting my SDS-PAGE. My marker seems to not be resolving fully with the last band coming out being 40kDa. I have attached a picture as an example.
My running conditions are:
10% separating gel and 4% stacking gel, both hand cast
150V for 1 hour 30 min
using the Bio Rad Mini Protean system
Any input would be greatly appreciated!