Stock solution is at 10 mM in DMSO - a lot of publications use this compound at a concentration higher than 10 uM but when I try, it systematically precipitates. Does someone have a trick? Maybe it reacts with a particular compound of the medium?
Try to use less concentrated stock in DMSO. Switch to another solvent (like methanol). But keep in mind this compound is not selective AMPK inhibitor. It hits a lot of other targets just as potently.
In some cases, the organic material will precipitate out of the solution. It is best to make the initial serial dilutions only in DMSO and then add the final diluted sample to your buffer or the incubation medium. The compound may be soluble in aqueous medium only at its working concentration.
Check out the attached book on "Introduction to Inhibitors" that I wrote recently. You will find a lot of useful tips in it.
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