Universal bacterial primers targeting a mRNA?

Hi, I'm looking for universal primers targeting a bacterial mRNA to quatify bacterial load by qPCR in a complexe mixture (eukaryote and prokaryote RNA). Ribosomal RNA being depleted, I absolutly...

22 February 2021 9,969 3 View

Is there anyone currently working with animal, 3D or silicone models for Lynch Syndrome?

Direct experiences with Biotechnological and Biomedical Approach? Therapies, long-term implications? I'm not looking for definitions but protocols and methods that you personally have made and...

03 December 2020 8,097 1 View

How to confirm that sf9 insect cells have been infected by Bacmid DNA and the P1 virus stock resulting got my GOI on it ?

Hi everyone. I transfected sf9 insect cells by my purified DNA bacmid. After 3 days post-transfection, I could not be able to see any cells infection signs; so I collected my P1 virus stock...

09 July 2018 3,047 5 View

TCR sequencing: is there any database where i can input a TCR epitope sequence and have the output be the specific pathogen that it corresponds to?

Hi there, I have evaluated TCR repertoire under different conditions and some amino acid sequences either shrink or expand with time. I found some databases where you input the organism and get...

18 January 2018 6,349 3 View

Which software do you use to analyse minirhizotron images? Which one is the most accurate to define the root length and diameter over time?

I would like to measure the root productivity of different mangrove trees. In this purpose, I will use the minirhizotron technique. I am currently running a lab experiment with mangrove tree...

05 November 2017 392 5 View

Can some one provides me with plasmid that can harbor two sgRNA, with a GFP for sorting transfected cells?

I would like to Knock out a gene in a cell line by using CRISPR/Cas9 method. In in order to increase the efficiency of the KO, I intend to use multiplex CRISPR/Cas 9 and for that, I need a plasmid...

29 March 2016 2,629 1 View

Why is my AMPK inhibitor (compound c/Drosomorphin) precipitating in the medium at 10uM?

Stock solution is at 10 mM in DMSO - a lot of publications use this compound at a concentration higher than 10 uM but when I try, it systematically precipitates. Does someone have a trick? Maybe...

10 March 2014 802 3 View

What is a suitable solvent to crystallize 2,3,4,6-Tetra-O-benzyl-D-galactopyranose?

I want to crystallize 2,3,4,6-Tetra-O-benzyl-D-galactopyranose (CAS Number 53081-25-7)? What would be a suitable solvent?

07 August 2012 8,284 15 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

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02 March 2021 3,060 3 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View