Universal bacterial primers targeting a mRNA?

Hi, I'm looking for universal primers targeting a bacterial mRNA to quatify bacterial load by qPCR in a complexe mixture (eukaryote and prokaryote RNA). Ribosomal RNA being depleted, I absolutly...

22 February 2021 9,969 3 View

How to confirm that sf9 insect cells have been infected by Bacmid DNA and the P1 virus stock resulting got my GOI on it ?

Hi everyone. I transfected sf9 insect cells by my purified DNA bacmid. After 3 days post-transfection, I could not be able to see any cells infection signs; so I collected my P1 virus stock...

09 July 2018 3,047 5 View

TCR sequencing: is there any database where i can input a TCR epitope sequence and have the output be the specific pathogen that it corresponds to?

Hi there, I have evaluated TCR repertoire under different conditions and some amino acid sequences either shrink or expand with time. I found some databases where you input the organism and get...

18 January 2018 6,349 3 View

Which software do you use to analyse minirhizotron images? Which one is the most accurate to define the root length and diameter over time?

I would like to measure the root productivity of different mangrove trees. In this purpose, I will use the minirhizotron technique. I am currently running a lab experiment with mangrove tree...

05 November 2017 392 5 View

Can some one provides me with plasmid that can harbor two sgRNA, with a GFP for sorting transfected cells?

I would like to Knock out a gene in a cell line by using CRISPR/Cas9 method. In in order to increase the efficiency of the KO, I intend to use multiplex CRISPR/Cas 9 and for that, I need a plasmid...

29 March 2016 2,629 1 View

Why is my AMPK inhibitor (compound c/Drosomorphin) precipitating in the medium at 10uM?

Stock solution is at 10 mM in DMSO - a lot of publications use this compound at a concentration higher than 10 uM but when I try, it systematically precipitates. Does someone have a trick? Maybe...

10 March 2014 802 3 View

What is a suitable solvent to crystallize 2,3,4,6-Tetra-O-benzyl-D-galactopyranose?

I want to crystallize 2,3,4,6-Tetra-O-benzyl-D-galactopyranose (CAS Number 53081-25-7)? What would be a suitable solvent?

07 August 2012 8,284 15 View

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02 March 2021 3,060 3 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

How to remove the residual PDMS on the silicon mold?

Incompletely cross-linked pdms are difficult to remove.

01 March 2021 798 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Mediation path analysis - Can I run two seperate models?

I am wanting to run a serial mediation model with 2 x IVs, 2 x MVs, and 3 DPs (all continuous). Is it problematic to run 2 different models for each IV, keeping the MVs and DPs the same in each...

27 February 2021 5,681 3 View

SEM or Multiple Regression for an analysis without Latent Variables?

Hello, I have three hypothesis to test, all with the same IV. I am testing if the effect of the IV on the first DV is mediated by awe. I am then measuring whether the effect of the IV on a...

26 February 2021 8,399 5 View

What software programs can directly simulate Additive Manufacturing Directed Energy Deposition (DED)?

I understand there are a lot of software programs that can simulate DED by going through specific modeling, simulation, and commands. For me, that was time consuming and results were not accurate....

24 February 2021 4,428 3 View

Are there lab markers/pens resistant to Roti-Histol?

Hi, I'm looking for a pen/marker to write on the smooth, transparent (non-frosted) part of microscopy slides which does not get washed away by alcohols and most importantly Roti-Histol. I want to...

24 February 2021 9,902 5 View