How to dissolve gluten for enzymatic hydrolysis?

Gluten is insoluble in water. It is composed of gliadin and glutenin. How to extract gliadin(soluble in aqueous alcohol) and how to use this extract for enzyme hydrolysis?

11 December 2016 7,257 1 View

Can 0.22 or 0.45 micron filter papers filter fungal spores?

I want to get rid of fungal spores in the protein sample extracted from fungi, so can these filter papers help me?

10 November 2016 9,987 8 View

Can beta-galactosidase act on substrates with alpha-galactose?

i.e.,can it break p-nitophenyl-D-galactopyranoside to release p-nitrophenyl? Can someone tell about the specificity of these enzymes?

03 April 2016 969 9 View

Which are the crystalline and which are the non crystalline regions in the XRD graph of cellulose?

For determining the degree of crystallinity, how are the crystalline and non crystalline regions considered?

05 June 2015 7,774 0 View

What are the degradative products of lignin acted upon by fungi?

Are these degradative products toxic to other organisms?

01 February 2015 4,809 3 View

Can fungi utilize the degradative products of lignin as their carbon sources?

Or it degrades lignin to reach cellulose or hemicellulose inside?

31 December 2014 2,770 4 View

How to select inoculum volume of non sporulating fungi for inoculating in solid state fermentation?

How much inoculum should be added per gram of solid substrate?

11 December 2014 776 7 View

Is enzyme activity measured in U/ml or IU/ml?

Can I get the difference between both of them?

09 October 2014 9,490 18 View

Is 0.1% of NH4Cl in BSS (Basal salt solution) sufficient enough to act as a sole nitrogen source?

Or is it required to add yeast extract and peptone in addition?

09 October 2014 7,373 2 View

For an cellulase assay, is it better to use DNS reagent with or without Rochelle salt?

If without Rochelle salt,then is it to be added at the end of heating period before cooling?

08 September 2014 5,200 6 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

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02 March 2021 3,060 3 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View