Rathinam Ramarajan

14 Questions 18 Answers 0 Followers

Questions related from Rathinam Ramarajan

How to dissolve gluten for enzymatic hydrolysis?

Gluten is insoluble in water. It is composed of gliadin and glutenin. How to extract gliadin(soluble in aqueous alcohol) and how to use this extract for enzyme hydrolysis?

12 December 2016 7,334 1 View

Can 0.22 or 0.45 micron filter papers filter fungal spores?

I want to get rid of fungal spores in the protein sample extracted from fungi, so can these filter papers help me?

11 November 2016 10,077 8 View

Can beta-galactosidase act on substrates with alpha-galactose?

i.e.,can it break p-nitophenyl-D-galactopyranoside to release p-nitrophenyl? Can someone tell about the specificity of these enzymes?

04 April 2016 1,016 9 View

Which are the crystalline and which are the non crystalline regions in the XRD graph of cellulose?

For determining the degree of crystallinity, how are the crystalline and non crystalline regions considered?

06 June 2015 7,816 0 View

What are the degradative products of lignin acted upon by fungi?

Are these degradative products toxic to other organisms?

02 February 2015 4,847 3 View

Can fungi utilize the degradative products of lignin as their carbon sources?

Or it degrades lignin to reach cellulose or hemicellulose inside?

01 January 2015 2,808 4 View

How to select inoculum volume of non sporulating fungi for inoculating in solid state fermentation?

How much inoculum should be added per gram of solid substrate?

12 December 2014 867 7 View

Why is 0.05 M or 0.1 M citrate buffer generally used for extracting cellulase from the substrates in SSF?

Why cant we use sodium phosphate buffer for extracting cellulase?Why are there many types of buffers with same pH?

12 December 2014 7,707 4 View

Is enzyme activity measured in U/ml or IU/ml?

Can I get the difference between both of them?

10 October 2014 9,782 18 View

Is 0.1% of NH4Cl in BSS (Basal salt solution) sufficient enough to act as a sole nitrogen source?

Or is it required to add yeast extract and peptone in addition?

10 October 2014 7,420 2 View

Is anybody familiar with the sensitivity of DNS method for glucose determination?

Is the DNS method for glucose determination sensitive below the concentration 40 µg/ml or is it suitable for higher concentrations only?

09 September 2014 6,051 4 View

For an cellulase assay, is it better to use DNS reagent with or without Rochelle salt?

If without Rochelle salt,then is it to be added at the end of heating period before cooling?

09 September 2014 5,276 6 View

Can anyone tell me what is the maximum enzyme activity (U/L) recorded till date for cellulases and hemicellulases by fungi?

08 August 2014 2,750 5 View

What is the difference between anaerobic and anoxic conditions?

Are both same? When an organism is in anoxic condition, does it shift to anaerobic mode of metabolism?

04 April 2013 3,493 18 View