How to dissolve gluten for enzymatic hydrolysis?

Gluten is insoluble in water. It is composed of gliadin and glutenin. How to extract gliadin(soluble in aqueous alcohol) and how to use this extract for enzyme hydrolysis?

11 December 2016 7,257 1 View

Can 0.22 or 0.45 micron filter papers filter fungal spores?

I want to get rid of fungal spores in the protein sample extracted from fungi, so can these filter papers help me?

10 November 2016 9,987 8 View

Can beta-galactosidase act on substrates with alpha-galactose?

i.e.,can it break p-nitophenyl-D-galactopyranoside to release p-nitrophenyl? Can someone tell about the specificity of these enzymes?

03 April 2016 969 9 View

Which are the crystalline and which are the non crystalline regions in the XRD graph of cellulose?

For determining the degree of crystallinity, how are the crystalline and non crystalline regions considered?

05 June 2015 7,774 0 View

What are the degradative products of lignin acted upon by fungi?

Are these degradative products toxic to other organisms?

01 February 2015 4,809 3 View

Can fungi utilize the degradative products of lignin as their carbon sources?

Or it degrades lignin to reach cellulose or hemicellulose inside?

31 December 2014 2,770 4 View

How to select inoculum volume of non sporulating fungi for inoculating in solid state fermentation?

How much inoculum should be added per gram of solid substrate?

11 December 2014 776 7 View

Why is 0.05 M or 0.1 M citrate buffer generally used for extracting cellulase from the substrates in SSF?

Why cant we use sodium phosphate buffer for extracting cellulase?Why are there many types of buffers with same pH?

11 December 2014 7,663 4 View

Is enzyme activity measured in U/ml or IU/ml?

Can I get the difference between both of them?

09 October 2014 9,490 18 View

For an cellulase assay, is it better to use DNS reagent with or without Rochelle salt?

If without Rochelle salt,then is it to be added at the end of heating period before cooling?

08 September 2014 5,200 6 View

How can we obtain a modification factor for creep parameters?

I want to obtain standard creep parameters of salt rock by creep parameters of rectangular samples. Is there a modification factor to convert creep parameters of rectangular (prismatic) rock salt...

01 March 2021 7,131 1 View

Anyone found any research papers on solar furnace design calculations (for solar concentration)?

I am thinking about working on solar furnace with heliostats that concentrate sunlight and heat molten salt, but currently no luck with research. Not a lot of papers based on the subject. (check...

01 March 2021 5,023 5 View

Heat exchanger with liquid Nitrogen as coolant?

I want to cool Natural Gas from 195 Kelvin to 138 Klevin in a heat exchanger by using liquid nitrogen as the coolant medium. When entering the heat exchanger, the liquid nitrogen will have a...

01 March 2021 6,450 5 View

Which transition metal salt precursor is best for doping? Why?

I would like to synthesize a transition metal doped TiO2 via solgel method. Hence, I would like to know which metal salt precursor to choose among Chloride, Nitrate, and Sulphate. A clear...

27 February 2021 4,285 3 View

Why do a weight decrease for thermal degradation in nitrogen gas condition?

Hi all The question is related to TGA(Thermogravimetric analysis). Thermal degradation easily understood but why do samples weight decrease? altough just binding is broked, the weight of...

26 February 2021 1,100 5 View

What are the saturated salt solutions that can give specific relative humidity of 35%, 65% and 95% for conditioning wood specimens?

Due to the unavailability of climate rooms, I want to carry out an experiment that require the use of certain saturated salt solutions especially those that can condition the wood specimen to 35%,...

25 February 2021 8,139 1 View

Steps to calculate activation energy by coats & redfern method?

I'm a chemistry student and I'm researching the kinetic degradation of mixed plastic waste. I'm having trouble calculating the activation energy by the coats & Redfern method. The data that...

24 February 2021 974 1 View

It is necessary to reflux the Schiff base and metal salts to obtain metal complex??

24 February 2021 9,166 3 View

How can I remove deep eutectic solvents from the medium?

Dear researchers, How can I remove deep eutectic solvents from the medium? You know, generally, deep eutectic solvents consist of ammonium salts and an acid group. Is there any alternative method...

22 February 2021 3,275 3 View

Physical-chemical formation of stalactites/stalagmites. what promotes the formation/growth of these structures?

I know, or think I do, the basic idea behind these natural structures, as from what I know they form due to water dripping from the top leaving a salt deposit behind. But started to wonder about...

22 February 2021 1,400 4 View