How to dissolve gluten for enzymatic hydrolysis?

Gluten is insoluble in water. It is composed of gliadin and glutenin. How to extract gliadin(soluble in aqueous alcohol) and how to use this extract for enzyme hydrolysis?

11 December 2016 7,257 1 View

Can 0.22 or 0.45 micron filter papers filter fungal spores?

I want to get rid of fungal spores in the protein sample extracted from fungi, so can these filter papers help me?

10 November 2016 9,987 8 View

Can beta-galactosidase act on substrates with alpha-galactose?

i.e.,can it break p-nitophenyl-D-galactopyranoside to release p-nitrophenyl? Can someone tell about the specificity of these enzymes?

03 April 2016 969 9 View

What are the degradative products of lignin acted upon by fungi?

Are these degradative products toxic to other organisms?

01 February 2015 4,809 3 View

Can fungi utilize the degradative products of lignin as their carbon sources?

Or it degrades lignin to reach cellulose or hemicellulose inside?

31 December 2014 2,770 4 View

How to select inoculum volume of non sporulating fungi for inoculating in solid state fermentation?

How much inoculum should be added per gram of solid substrate?

11 December 2014 776 7 View

Why is 0.05 M or 0.1 M citrate buffer generally used for extracting cellulase from the substrates in SSF?

Why cant we use sodium phosphate buffer for extracting cellulase?Why are there many types of buffers with same pH?

11 December 2014 7,663 4 View

Is enzyme activity measured in U/ml or IU/ml?

Can I get the difference between both of them?

09 October 2014 9,490 18 View

Is 0.1% of NH4Cl in BSS (Basal salt solution) sufficient enough to act as a sole nitrogen source?

Or is it required to add yeast extract and peptone in addition?

09 October 2014 7,373 2 View

For an cellulase assay, is it better to use DNS reagent with or without Rochelle salt?

If without Rochelle salt,then is it to be added at the end of heating period before cooling?

08 September 2014 5,200 6 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

Does anyone know to clarify these changes?

01 March 2021 1,290 2 View

How can I make stacked bar graph with scatter plot?

I want to make a stacked bar graph with showing each data value as dots. I tried with graph pad prism, version 6, but could not succeed. Does anyone have tried before making such graphs?

01 March 2021 9,383 1 View

In an eye with Tyndall, does each dot correspond to an individual cell?

When you observe the presence of Tyndall in an inflamed eye: Does each of the small floating dots correspond to an individual cell or to an aggregate of cells? Does a conventional slit lamp have...

27 February 2021 6,326 2 View

How to calculate the crystallinity of cellulose film?

27 February 2021 2,753 4 View

What could be the reason for not having further low chi square value (better agreement) in my refinement result?

I have tried every combination of parameter changes and after that i have a poor chi square value. I can't get it lower any further. I know there have been a lot of discussion about Rietveld...

26 February 2021 8,480 3 View

What could be reason for getting such type of graph for complex impedance in case of polymer blend films?

Its effect appears in real part of ac conductivity also. Can anyone suggest any reason behind it?

26 February 2021 3,487 3 View

Can anyone share his experience regards Smoothing in origin?

I am working on TiO2 composite material and the results of XRD have a lot of small/junk peaks and while I apply a smooth filter in Origin then the other characteristics peaks disappear so anyone...

26 February 2021 8,183 3 View