Why cant we use sodium phosphate buffer for extracting cellulase?Why are there many types of buffers with same pH?
The working pH range of citrate buffer is from 4.0 to 5.5. Since most of the cellulases from Fungi when grown under SSF conditions are acidic in nature, it is best to use citrate buffer for extraction and activity checking as well. Phosphate buffers work best around neutral pH, so they are generally not used for extraction of cellulases.
Citrate phosphate buffer or citrate buffer both can serve the purpose. The only advantage using Citrate phosphate buffer ( Citric acid (0.1M) ; Na2HPO4 (0.05 M) ) is that it has much broader pH range from 3.5 to 6.5. Now it depends on you which buffer you wish to use. If you know that your cellulases act best at 4.5 then go for citrate buffer and if you have no idea of the exact pH working range use citrate phosphate buffer
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