Deepti Agrawal

10 Questions 126 Answers 0 Followers

Questions related from Deepti Agrawal

What is the best method of compositional analysis for lignocellulosic feedstocks having >10% ash content ?

In our latest study (Comparative assessment of sugarcane bagacillo and bagasse at... ) and even by the study conducted by Sluiter et al (https:// doi. org/ 10. 1021/ jf100 8023), it has been...

01 June 2023 2,552 0 View

How relevant is FPU assay in case of new generation LPMO containing cellulase cocktails ?

Filter paper activity (FPU) was devised by Prof. T.K Ghose for measuring overall cellulase activities. However, the most critical point of this assay was the release of exactly 2mg glucose from...

27 February 2020 5,711 0 View

How experimental evidences can be provided for better enzymatic hydrolysis of wet against dry pretreated lignocellulosic biomass?

During enzymatic saccharification drying of pretreated biomass normally impedes cellulose hydrolysis as compared to its wet counterpart. What analytical techniques can be employed to justify the...

02 January 2020 7,397 3 View

Why does the volume of aqueous fraction increase during Lactic acid extraction while using TBP and Chloroform as extractant and diluent respectively?

We are trying to extract Lactic acid (LA) from fermentation broth. During the screening of various diluents for LA extraction using Tributyl phosphate (TBP) as the base case, we are observing an...

24 September 2019 6,820 0 View

During growth studies with Scenedesmus higher growth is seen in Tricine buffered medium as compared to HEPES. What is the plausible explanation ?

Why growth in Tricine buffered medium is better to traditionally HEPES buffered medium for Scenedesmus at same pH? Even good buffers like MOPS and Gly glyc are not performing. What could be be...

01 March 2017 5,045 3 View

How can one detect LPMO activity in the cellulase enzyme extracts ?

Recently it has been proven that GH61 group of enzymes belonging to CaZy group has shown to play crucial role in enzymatic saccharification. How can one detect LPMO activity in the crude cellulase...

21 February 2017 1,813 1 View

How cellobiase/beta glucosidase can be exactly traced in a zymogram since its substrate and products both are water soluble ?

We are studying the secretion pattern of Cellulase enzyme system in Penicillium sp. for Lignocellulosic biomass hydrolysis. It is very convenient to trace the endoglucanase in zymograms by...

29 September 2016 415 5 View

Why is lyophilization the preferred way of drying oleaginous microalgae?

In numerous papers I have I read that before the extraction of lipids the oleaginous microalgae was dried via lyophilisation process. Will oven drying of microalgae affect the quality ( oxidation...

30 July 2015 9,439 4 View

Does anyone have a protocol for a Malic Enzyme assay in Oleaginous yeast?

Using Malate as a substrate I am unable to get a linear graph (Increase in optical density at 340 nm) when I am performing Malic enzyme assay in a time course mode. What should be the optimum...

02 May 2014 8,430 9 View

Relevance of FPU assay in case of new generation LPMO containing cellulase cocktails

Filter paper activity (FPU) was devised by Prof. T.K Ghose for measuring overall cellulase activities. However, the most critical point of this assay was the release of exactly 2mg glucose from...

01 January 1970 7,623 0 View