Rathinam Ramarajan

14 Questions 13 Answers 0 Followers

Questions related from Rathinam Ramarajan

Can beta-galactosidase act on substrates with alpha-galactose?

i.e.,can it break p-nitophenyl-D-galactopyranoside to release p-nitrophenyl? Can someone tell about the specificity of these enzymes?

15 April 2016 4,902 9 View

Why is FPase considered as a measurement of the total cellulase activity?

How is it related to CMCase and avicelase? Is there any theoretical relationship between all these?

11 March 2015 1,938 3 View

Which substrate will give higher enzyme activity for cellulase?

Simpler substrates like carboxymethyl cellulose(CMC), avicel or substrates like rice straw, sugarcane bagasse can give cellulase with better activity. Rice straw and similar substrates have lignin...

04 March 2015 6,215 3 View

What are the degradative products of lignin acted upon by fungi?

Are these degradative products toxic to other organisms?

27 February 2015 653 3 View

Can pretreatment and saccharification happen simultaneously if lignocellusic biomass is the substrate acted upon by fungi?

When a fungi possess both lignin degrading as well as cellulose degrading activity, then which activity will be expressed more if lignocellulosic substrate is given?

27 February 2015 2,295 4 View

Can fungi utilize the degradative products of lignin as their carbon sources?

Or it degrades lignin to reach cellulose or hemicellulose inside?

01 January 2015 7,324 4 View

Why is 0.05 M or 0.1 M citrate buffer generally used for extracting cellulase from the substrates in SSF?

Why cant we use sodium phosphate buffer for extracting cellulase?Why are there many types of buffers with same pH?

16 December 2014 4,764 3 View

How can I calculate the maximum theoretical yield of total glucose and xylose from lignocellulosic biomass?

Since there is no specific molecular weight for lignocellulosic, what is the maximum theoretical yield of total glucose and xylose from lignocellulosic biomass calculated?

09 December 2014 1,032 4 View

What is the extinction coefficient of glucose?

While calculating cellulase or xylanase enzyme activity, is it preferable to use the extinction coefficient of glucose or to use the standard factor from the standard graph of glucose?

01 December 2014 4,371 3 View

Why is it that only endoglucanases are known as CMCase?

Does CMC (carboxy methylcellulose) require all the three groups of cellulases (namely endoglucanases, cellobiohydrolases and b-glucosidase) for degradation? Or, does it only require endoglucanases...

28 October 2014 923 4 View

Is enzyme activity measured in U/ml or IU/ml?

Can I get the difference between both of them?

07 October 2014 8,416 20 View

Is anybody familiar with the sensitivity of DNS method for glucose determination?

Is the DNS method for glucose determination sensitive below the concentration 40 µg/ml or is it suitable for higher concentrations only?

25 September 2014 1,962 3 View

For an cellulase assay, is it better to use DNS reagent with or without Rochelle salt?

If without Rochelle salt,then is it to be added at the end of heating period before cooling?

24 September 2014 3,703 6 View

Can anyone tell me what is the maximum enzyme activity (U/L) recorded till date for cellulases and hemicellulases by fungi?

05 August 2014 8,339 5 View