I am currently working with a specific type of cells called mouse alveolar macrophages (MH-S) in my experiments. During cytometry analysis, I consistently observe two distinct populations when I separate the single cells, which is also reflected in the sharp intensity of these two populations. Despite my efforts to investigate the cause, I consistently obtain the same result. Since these cells grow in both suspension and adherent forms, I attempted to separate the populations and determine if the two populations are due to different cell types (suspension and adherent). I reduced the cell density and tested different conditions, but I am unable to comprehend the nature of the second population.
In order to gain further insight, my plan is to examine the suspension and adherent cells using surface markers to determine if they are indeed distinct but I he FSC-H VS FSC-A parameters.
In order to gain further insight, my plan is to examine the suspension and adherent cells using surface markers to determine if they are indeed distinct but I have doubts yet.
Concerning the possibility of cross-contamination, I conducted repeated experiments using different batches of cells, yet the results remained consistent.
I would greatly appreciate your opinion and insights regarding this matter.
Best regards,
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