the gel was stained using Sypro Ruby reused 1x
What Will be the Impacts of the New Satoshi Nakamoto Post On Twitter on the Future Research Lines? Satoshi Nakamoto posted on Twitter after five years of hiatus on October 5, 2023. The latest...
16 October 2023 8,076 2 View
I've noticed that some publishers and journals have a very fast review process, with high acceptance rates for articles regardless of their novelty and quality, as long as you pay APCs. Some of...
15 June 2020 9,024 8 View
what is the fastest method to measure 100 ppm of the heavy elements in the solid food products, plastic,and water? XRF can, but what is the best company and model? neutron activation can be used...
29 August 2018 9,025 11 View
Hello, i ordered couple of isotopic labeled peptides (13C/15N) for absolute quantification of proteins. The first measurements show that the light one from protein standard and the heavy one do...
20 September 2016 2,836 5 View
I read in many references, but I can't connect between ideas! "blood pressure is higher in men than in women at similar ages. After menopause, however, blood pressure increases in women to levels...
14 April 2016 9,499 10 View
I read in a reference that: "Each person would be heterozygous for 24,000-40,000 non-synonymous (amino acid altering) substitutions". Could you please tell me what does that mean?
29 March 2016 8,718 3 View
I am working on a PCR. I did gradient PCR to make sure that the annealing temperature mentioned in the reference study is the best temperature for annealing. I found there were lots of secondary...
26 December 2015 8,202 3 View
I am working on a research about association between hypertension and only one SNP. After statistical study, i found there is No association between genotype and phenotype. how can i explain and...
26 December 2015 6,280 6 View
I am looking for theory Information about angiotensin II receptors and about their genes, PDF or electronic books. Can you help me? Thanks
26 December 2015 2,338 2 View
I wonder if we have the problem of pilot contamination in LTE especially in the case of TDD, as the users also need to send the pilots to the base station, so we still need a large number of...
07 December 2015 9,184 2 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,480 3 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...
06 August 2024 3,130 2 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...
02 August 2024 6,735 1 View
Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...
31 July 2024 2,406 6 View
We are working on biopolymeric hydrogels. Our system is highly viscous and sticky, and the gel formed are high in strength. We are unable to use pH electrode and pH strip. Please suggest an easy...
30 July 2024 942 2 View
I am using CuBr/THPTA for a click reaction in total cell lysates. I am facing issues with my protein sample in non-reducing SDS-PAGE where it's not migrating properly and most of it remains at the...
29 July 2024 950 4 View
