What happens when silver nanoparticles (Aggregate of Ag0) are released into the environment? Can it be change into the Ag3+ or remain as same (Ag0)?

I have synthesized silver nanoparticles form green route. While washing the glassware, the nanoparticle are released which must be entering into the environment. I want to know that is there any...

31 December 2018 1,876 14 View

What are the components of a sensor? How it works?

Hello, This is Dr. Vijay, I am want to design a sensor for different heavy metal ions. I want to know the basic idea behind it. Thank you.

11 December 2018 4,926 0 View

Why the gold nanoparticles synthesized from leaf extract get negatively charged?

I have synthesized gold nanoparticles from aqueous leaf extract of Croton bonplandianum. The zeta potential result showed it is negatively charged. Please explain the exact reason of this...

05 June 2018 4,222 9 View

How to calculate the concentration of carbon quantum dots ?

I have prepared carbon quantum dots by taking 200 mg precursor in 200 mL distilled water. Its very tough to precipitate the carbon quantum dots by centrifugation. It is in solution form. How can I...

31 December 2017 3,630 4 View

How can I calculate the concentration of gold nanoparticles in suspension ?

Hello all,I have synthesized the AuNPs using 1.2 mM auric chloride salt. How can I know its concentration in suspension form?

06 July 2017 3,837 5 View

Why the size and anisotropic nature of the green synthesized AuNPs increased with the increase in metal ion concentration?

The green synthesis of gold nanoparticles was investigated using different metal ion concentration at a fixed inoculum dose and reaction incubation time. It was observed that the size of the...

11 December 2016 813 8 View

What should I write in discussion about the 2D, 3D and average roughness profile of nanoparticles?

09 October 2016 8,193 2 View

Which standard additional technique should be applied for the determination of Iron (III) in terms of validation?

The colorimetric detection of Iron (III) was carried out using AgNPs synthesized by plant extract. Different concentrations of Iron (III) were added into 1 mL solution of AgNPs and the change in...

08 September 2016 6,606 5 View

Why the peak of rGO did not appear in the XRD spectra of Ag-rGO-PANI?

The composite of Ag-rGO-PANI was prepared and characterized through XRD technique. When the XRD spectra of rGO and PANI were taken separately, a characteristic band appeared but these did not...

08 September 2016 7,268 7 View

What differences could be seen in pre and post annealed nanoparticles when characterized through XRD ?

I have synthesized the AgNPs using plant extract and annealed it at 300 degree Celsius. The pre annealed sample was also characterized by XRDNow what differences could be seen?

05 June 2016 3,424 16 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

What is the best way to keep an anaerobic chamber anoxic in the absence of hydrogen?

I'm a graduate student who works with an anaerobe, so I often work in an anaerobic chamber. I've been working on an assay that involves the steady-state of the quinone/ quinol pool. Anoxic...

03 March 2021 2,971 1 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Can chitosan alone can act as a reducing agent for Cu(0) nanoparticle sythesis?

[Synthesizing Cu nanoparticles without using chemical reductants]

01 March 2021 8,359 1 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What are the new 2D nanomaterials were used for Silver nanoparticles stabilization?

In the stabilization of AgNPs are vital in current research interest. However, some of the 2D nano-materials like graphene oxide, MoS2, etc... previously used to stabilize it. But still they shown...

01 March 2021 2,207 3 View