add 4ul human blocking primer, but human DNA still come out, there is a SPC3 in 3 end of the primer
how to extract DNA from amphibian eggs? as there is little tissue in the egg.
06 July 2018 8,833 0 View
an article classify islands as “small” islands (0.25 to 0.9ha), “medium” islands (4 to 12 ha), “large” islands ($150 ha),
05 June 2017 5,512 2 View
sample was kept in absolute ethanol, if i can use Proteinase K deal with this sample?
04 May 2017 3,427 1 View
Herder, J.E., Kranenbarg, J., De Bruin, A., Valentini, A. Op jacht naar DNA Effectief zoeken naar grote modderkruipers. Visionair 8–11.
04 May 2017 3,339 1 View
its a perssad in blocking primer
01 January 1970 6,503 2 View
my PCR product only 3ng/ul
01 January 1970 1,668 3 View
a part of 12s DNA
01 January 1970 1,330 0 View
i don't want to download the whole data from NCBI, just want Vertebrate 12s mDNA
01 January 1970 8,530 1 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
How to design VN primer to attach with universal reverse primer
05 August 2024 2,116 3 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View
Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...
03 August 2024 3,371 3 View
I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?
02 August 2024 3,143 4 View
Molecular docking software/ websites?
02 August 2024 8,704 7 View