What does 'i' mean in SpacerC3i?

More Wenhao Li's questions See All

How to extract DNA from amphibian eggs?

how to extract DNA from amphibian eggs? as there is little tissue in the egg.

06 July 2018 8,833 0 View

How to classify islands by size?

an article classify islands as “small” islands (0.25 to 0.9ha), “medium” islands (4 to 12 ha), “large” islands ($150 ha),

05 June 2017 5,512 2 View

Why human blocking primers dosent work?

add 4ul human blocking primer, but human DNA still come out, there is a SPC3 in 3 end of the primer

04 May 2017 508 0 View

Can absolute ethanol suppress the activity of Proteinase K?

sample was kept in absolute ethanol, if i can use Proteinase K deal with this sample?

04 May 2017 3,427 1 View

Does this article have an English version?

Herder, J.E., Kranenbarg, J., De Bruin, A., Valentini, A. Op jacht naar DNA Effectief zoeken naar grote modderkruipers. Visionair 8–11.

04 May 2017 3,339 1 View

How to improve the concentration of PCR product?

my PCR product only 3ng/ul

01 January 1970 1,668 3 View

Where is 12S-V5 region

a part of 12s DNA

01 January 1970 1,330 0 View

How to build a Vertebrate 12s mDNA database

i don't want to download the whole data from NCBI, just want Vertebrate 12s mDNA

01 January 1970 8,530 1 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

05 August 2024 2,116 3 View

I'm optimizing a tetra-ARMS PCR protocol with amplicon sizes: 165bp, 123bp and 93bp. Why, in the gel, only 165bp is visible while I'm expecting all 3?

It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...

01 August 2024 4,673 4 View

PCR showing no bands - Master mix and primers didn't mix?

Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...

31 July 2024 2,406 6 View

How to retain amplicon yield after Ampure bead cleanup, following a 2-stage PCR protocol?

Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...

30 July 2024 841 2 View

Culture fibroblasts with or without FGF2?

I'm new to primary human fibroblast culture and I'm studying aging using cells from old and young donors. Some research groups have used media supplemented with FGF2, so I cultured cells in the...

29 July 2024 3,030 2 View

Do you mix and add all primers together in a singel PCR reaction (4 total primers for 2 mutations) when using Agilent's multi-site mutagenesis kit?

I want to introduce 2 mutations using Agilent's multi-site mutagenesis kit, I designed the primers using their online tool and it gave me 2 sets for primers (1 set for each mutation) so I was...

25 July 2024 6,517 3 View

Can I please ask why my samples from anaerobic bioreactor giving me different size PCR product even after multiple runs?

Hi everyone, I have extracted DNA from a biogas bioreactor using Qiagen kit and prep cDNA library then used this library as template to optimize primers for qPCR (taken from papers). Some of the...

23 July 2024 1,329 5 View

Are there always been barcodes, apapters and primer sequences in the FASTQ files of NGS?

Hello researchers, Sorry for my stupid question. I am learning the QIIME2 workflow for analyzing some 16s amplicon NGS fastq data. I found a very nice paper with data and code public available...

20 July 2024 5,405 2 View