how to extract DNA from amphibian eggs? as there is little tissue in the egg.
an article classify islands as “small” islands (0.25 to 0.9ha), “medium” islands (4 to 12 ha), “large” islands ($150 ha),
05 June 2017 5,512 2 View
add 4ul human blocking primer, but human DNA still come out, there is a SPC3 in 3 end of the primer
04 May 2017 508 0 View
sample was kept in absolute ethanol, if i can use Proteinase K deal with this sample?
04 May 2017 3,427 1 View
Herder, J.E., Kranenbarg, J., De Bruin, A., Valentini, A. Op jacht naar DNA Effectief zoeken naar grote modderkruipers. Visionair 8–11.
04 May 2017 3,339 1 View
its a perssad in blocking primer
01 January 1970 6,503 2 View
my PCR product only 3ng/ul
01 January 1970 1,668 3 View
a part of 12s DNA
01 January 1970 1,330 0 View
i don't want to download the whole data from NCBI, just want Vertebrate 12s mDNA
01 January 1970 8,530 1 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...
06 August 2024 666 4 View
Palmitic acid presence in aqueous fraction
05 August 2024 8,624 4 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...
03 August 2024 4,725 2 View