How to extract DNA from amphibian eggs?

how to extract DNA from amphibian eggs? as there is little tissue in the egg.

06 July 2018 8,740 0 View

How to classify islands by size?

05 June 2017 5,360 2 View

Why human blocking primers dosent work?

04 May 2017 411 0 View

Can absolute ethanol suppress the activity of Proteinase K?

sample was kept in absolute ethanol, if i can use Proteinase K deal with this sample?

04 May 2017 3,330 1 View

Does this article have an English version?

04 May 2017 3,222 1 View

What does 'i' mean in SpacerC3i?

01 January 1970 6,423 2 View

How to improve the concentration of PCR product?

my PCR product only 3ng/ul

01 January 1970 1,586 3 View

Where is 12S-V5 region

a part of 12s DNA

01 January 1970 1,240 0 View

How can I retrieve FASTA sequence from gene ID or UID of a Gene ? I have GeneBank IDs ?

I treid to use Entrez Batch, but it redirects me to NCBI site. Is there any script of Linux or Python through which I can download Fasta sequence of list of gene ID ?

21 February 2021 7,783 12 View

MHC Rabbit Genome Annotation? Domain annotation of each MHC Rabbit Gene?

I want to find a database(s) or a method where I can find and bulk download all the rabbit MHC genes and domain annotation. In humans using Ensembl, all MHC genes have names and domains but in...

28 January 2021 7,722 2 View

Once I annotate a genome with PROKKA, where do I uplaod it?

I have annotated a genome successfully with PROKKA. I can upload the genome to NCBI, but I cannot upload the annotation. PROKKA does not produce files in the correct format, and I cannot find any...

29 December 2020 3,976 3 View

How can I design primers of some micro RNAs online ?

How to use NCBI to design primers for specific Micro RNAs for quantitative analysis and how to check the efficacy of the selected primer before working

24 December 2020 180 3 View

How can I know which sequence is the correct one if there are lot of predicted sequence in database for my target gene?

Right now, I want to design qPCR primer for my target gene. But that gene has 21 predicted mRNA sequence in the NCBI database. Most of the sequence names are same but only different location...

23 December 2020 5,531 12 View

What really is "Prechordal Plate"?

There is too much discrepency among the textbooks of vertebrate embryology. There are two interpretations of the term "Prechordal plate" in different books: It is the cranial most part of the...

24 November 2020 8,537 1 View

Which is the best mitochondrial genome annotation tool? Can anyone provide the list?

I have sequenced the mitochondrial genome of some moths species. I want to annotate and the sequence will submit in the NCBI.

19 November 2020 8,849 1 View

Anyone an expert in algae in Victoria, Australia?

I found this (very pretty) algae (see picture) in a bore-fed and suburban catchment run-off fed, human made lake (Newport Lakes) in Newport, Victoria, Australia. I'm a vertebrate zoologist with a...

09 November 2020 7,587 3 View

Is it possible to get genomic context data on bulk of accessions?

I'm trying to build local gene synteny to identify homologs between different species. To do that, I've been accessing each individual gene of interest, going into gene view in NCBI and...

04 November 2020 9,038 2 View

Why will only one of three genes amplify CRISPR-Cas9 triple knock out DNA preps?

Working to genotype CRISPR-Cas9 triple mutants. One of the three genes amplifies very well, the other two won't. I have tried three different primer pairs for each of the two genes that won't...

12 October 2020 2,429 3 View