Radek Machan

4 Questions 59 Answers 0 Followers

Questions related from Radek Machan

What can cause laser beam polarisation fluctuations?

Dear colleagues, We have observed large fluctuations in laser power in our setup and traced them down to fluctuations in laser beam polarisation. Attached is a schematic of the relevant part of...

15 January 2021 7,899 4 View

What is the best way to identify whether a certain substance is present in a mixture based on Raman spectra?

Dear Colleagues, our imaging facility is entering the field of Raman micro-spectroscopy and we've been approached by a group who want to detect the presence of certain compounds (about 10...

09 January 2021 1,094 5 View

Do microtubules wind around each other?

We have recently taken some super-resolved images of live CHO-K1 cells expressing eGFP-tubulin. Some of the microtubule strands appear in the super-resolved image to be actually two tubules...

10 March 2016 4,113 2 View

Can anyone recommend a water cooling system for a sCMOS camera?

We would like to use water cooling with our sCMOS camera (Hamamatsu Orca Flash 4) to avoid any vibrations caused by the fan. The user manual recommends a standard water circulator bath, which...

05 November 2014 6,074 5 View