When transcribing RNA from a DNA strand, do all resulting RNA strands possess a triphosphate group? What is the mechanism for this to happen? When is it necessary to add a cap on the 5' end?
I am looking for journals that admit the publication of results from applying the IUCN Standard for designing and monitoring Nature-based solutions. Many thanks for considering my request.
23 July 2024 6,444 2 View
I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...
22 July 2024 5,953 6 View
What cell proliferation assay do you suggest? cyQUANT from thermo Fischer has good reviews, a lot of good articles for the kit from Abcam. and Promega kits look easy and straight forward.
21 July 2024 1,554 2 View
How to assign allele numbers and sequence type after amplifying housekeeping genes by PCR for genotyping of S. pneumoniae strains by MLST using bioedit ?
19 July 2024 5,239 2 View
we want to produce chocolate milk industrally .Dose anyone have a specific stabilizer formula for this pourpose?
10 July 2024 3,791 1 View
Are there any techniques at ore exploration that could replace the compass-tape measure of mineralized structures?
14 June 2024 3,254 2 View
I tried western blot targeting TTF-1 (Thyroid transcription factor-1) also known as Nkx2.1. I tested two primary antibodies, one is Invitrogen, Nkx2.1 Monoclonal Antibody (8G7G3/1) and the other...
29 May 2024 3,517 3 View
Hi everyone, I try to do a simulation about heat transfer of supercritical hydrogen in a cooling channel. Properties of H2 is obtained from NIST and displayed by an UDF function. I used the k-w...
16 May 2024 4,076 2 View
I am planning to extract DNA with QIAamp Mini DNA extraction kit (Qiagen) from DNA Shield microbiome preserved samples. My samples contain small amount of cell, so my goal is to reduce the loss of...
14 May 2024 2,291 2 View
14 May 2024 7,178 3 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View