Sara Sabina Sánchez

3 Questions 1 Answers 0 Followers

Questions related from Sara Sabina Sánchez

What are some characterization techniques for RNA?

I am working on the transcription of an RNA strand. More than knowing all the characteristics of my RNA, I would like to know if the transcription was successful or not. What methods can be used...

09 March 2023 4,639 2 View

Why does transcribed RNA have triphosphate group on the 5' end?

When transcribing RNA from a DNA strand, do all resulting RNA strands possess a triphosphate group? What is the mechanism for this to happen? When is it necessary to add a cap on the 5' end?

09 March 2023 7,690 0 View

How to produce a triphosphate (3p) motif by in vitro RNA transcription?

I want to perform an in vitro RNA transcription to obtain an RNA strand with a 3p (Triphosphate) motif added at the 5' end. I have found some protocols but most only use a DNA template and the...

12 October 2022 5,495 1 View