generally after the addition of protein the peak current of molecule decreases.. but in my case i observed that the peak current was increased.
Peak current or peak absorbance?
I am trying to estimate ROS in insect tissue. Is it possible to do it using DCFDA
18 June 2024 6,954 0 View
A survey site of mine is an agricultural plot that for 4 months is used for growing paddy, for the next 4 months it is a meadow and for 4 months it is used for growing vegetables. Can all these be...
14 December 2023 7,049 1 View
I need a hard copy of the field guide for Odonate of Maharashtra, India.
05 December 2023 8,057 2 View
Odonates tend to glide, fly, hover, and move frequently. During transect surveys, it is possible to count one individual more than once if it flies back and forth. Is there any method (statistical...
30 October 2023 6,829 4 View
Inputs-Laser power Layer thickness Scan speed Hatch spacing Output- Relative density, UTS, Hardness, Yield strength, Surface roughness , % Elongation
21 October 2023 4,770 1 View
And please explain when we observe quantum confinement going below 10nm.
27 June 2023 9,884 0 View
Hi all , so I have to make 5% dialyzed FBS from the normal FBS which we use for cell cultures, does anyone have the protocol for 5% dialyzed FBS preparation?
18 June 2023 6,392 0 View
So we have to do process the samples for chip seq and these are the cells of PANC - 1. My colleague fixated these cells 2 months back and we were practicing for running the samples. So we...
25 May 2023 3,509 5 View
I am using Quercetin for some antimicrobial assay and it has proven to be an antimicrobial agent against various pathogens. But my research is being limited due to the poor solubility of Quercetin...
21 May 2023 5,506 3 View
I have data for 5 sets of 10 replicates including Negative control. Using Graphpad PRISM, how to get the Pearson r and P value?
21 May 2023 9,190 1 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
I'm having problems with ion exchange chromatography. After applying my sample (a fluorescent protein), a preferential path inevitably forms. The matrix I use is DEAE-Sephacel. Could someone...
27 July 2024 6,605 0 View
I use 0.5% sarkosyl to solubilise recombinant proteins contained in Inclusion bodies of E. coli. sarkosyl very effeciently solubilizes proteins from Inclusion bodies and I am trying to use...
25 July 2024 5,001 2 View
"The Correlation between Nutrition and Transport Mechanism under Abiotic Stress in Plants: A Comprehensive Review" by Muhammad Saleem, Jianhua Zhang, Muhammad Qasim, Rashid Iqbal, and Li Song,...
23 July 2024 9,109 0 View
Good morning, I am trying to produce a recombinant protein. Unfortunately I one of the two restriction enzymes that I use do not cut at the end of the MCS. After the digestion 15 nucleotides of...
17 July 2024 2,787 4 View
My protein is a transmembrane protein, therefore i need to construct a transmembrane using CHARMM-GUI. Can someone please suggest me a tutorial for the same.
24 June 2024 5,044 1 View
Hi, may I know I want to fuse my protein with affinity tag for protein purification in Expi293, how can I determine and visualise how does a N or C terminal tag affect my proteins (structure,...
24 June 2024 8,622 6 View
Hello esteemed scientists, I am endeavoring to increase the yield of my recombinant protein. It is a 120 kDa protein tagged with MBP and features three intrinsically disordered regions with pI...
12 June 2024 9,616 8 View
Hi everyone, I've been having difficulty in purifying a protein. It is a beta barrel, integral membrane protein. My protein is expressed and purified recombinantly. My target protein is running...
30 May 2024 7,604 3 View