What will be the impact on Socio-Economic activities of man after the end of COVID-19 Pandemic?

I am very much thankful of the experts, researcher, and thinkers for very thought full discussion on my previous question on Role of Geography on COVID-19 Pandemic. Now I am keeping another very...

19 October 2020 6,552 4 View

How to model deformable metal powder?? How to define gap between the particles?? Which modeling software can be used??

We need to model deformable metal powder. It will be further used in simulation process.

30 September 2020 9,329 7 View

I am keeping the Question for discussion, What is the role of Geography in the study of COVID-1 pandamic?

Please give your opinion and suggestions

10 August 2020 3,990 19 View

How to add different colors to a pseudo- colour image using Fiji ImageJ?

I have a grayscale image of which the original RGB image was formed by combining Green and Red channels which represent different types of cells (live/dead). Splitting the channels in RGB mode is...

11 March 2020 1,616 3 View

How to convert SAR Image Data(Radarsat or sentinel-1A) Compatible to Matlab?

I am trying to implement log normal mixer model algorithm for ship detection using synthetic aperture radar images in Matlab. In this I need to give SAR image data (complex data) as an input to...

21 February 2020 4,450 3 View

How do I force material studio to stop or break the current command in progress before it crashes?

Something equivalent to Ctrl+C in Matlab to break the code. Thank you in advance.

22 January 2020 4,545 2 View

Scope of use of zeolite and bio char in dryland agriculture?

Now a days use of bio char to replace organic fertilizer is increased and discussed very fluently in scientific forum. Also to conserve the moisture is zeolite is beneficial in dryland that also...

31 December 2019 3,540 1 View

What is photosensitizer and photocatalyst are they same concept or different ?

The material like tris bipyridyl ruthenium trichloride II [Ru(bipy)3]Cl2 or dyes such as rose bengal,eosin are photocatalyst or photosensitizers?

27 December 2019 7,061 4 View

What is difference between classical semiconducting nanocomposites as a photocatalyst and organic dye based photocatalyst ?

Are the concept of valence band and conduction band applies for the dye based photocatalyst like semiconductor based material if it is then how?

27 December 2019 3,464 3 View

If doing part time PhD from a University and publishing PhD research with affiliation of working place. Can it be counted as PhD research?

If anyone is doing part time PhD from a University and publishing his PhD research work with affiliation of working place as using resources there. Can his/her published paper be counted as part...

25 November 2019 1,420 2 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View

Is it possible to quantify recombinant protein in the supernatant using SDS PAGE ?

I would like to quantify a recombinant protein (with native signal sequence) secreted by Pichia pastoris using the SDS PAGE. Since my standard protein (plant origin) is not pure, I would like to...

07 February 2021 3,880 9 View

How to measure the concentration of a labeled protein with no tryptophan or tyrosines?

Hey I'm trying to measure the concentration of a protein I've purified and then labeled with Atto 647 flourescent dye. The protein has no tryptophans or tyrosines (although does have...

04 February 2021 4,482 4 View

How to prepare protein samples in EMSA assay?

Recently l was studying EMSA assay, but l didn't find any detailed information about protein sample. we can use two kinds of protein, including crude protein extracts and recombinant proteins. but...

02 February 2021 10,047 3 View

Difference between assessing EC50 in presence of physiological levels of human serum albumin and alpha1 acid glycoprotein vs. % human serum?

01 February 2021 1,825 1 View

Alpha synuclein lack structurw (i.e some of the helix part goes into turn ), any cause behind this?

I am trying to simulate alpha synuclein (1xq8.pdb) in GROMACS using GROMOS53a6 ff. I have run it for 200 ns. At the end of simulation alpha helix protein losses its helical structure. I am not...

31 January 2021 4,285 3 View

How to increase the lower detection limit of ELISA?

Hi all, I am trying to establish an ELISA method for the quantification of a recombinant protein injected into mice. In the meantime I am trying to make calibration curves with the purified...

27 January 2021 8,702 3 View