Hi all , so I have to make 5% dialyzed FBS from the normal FBS which we use for cell cultures, does anyone have the protocol for 5% dialyzed FBS preparation?
I am trying to estimate ROS in insect tissue. Is it possible to do it using DCFDA
18 June 2024 6,954 0 View
A survey site of mine is an agricultural plot that for 4 months is used for growing paddy, for the next 4 months it is a meadow and for 4 months it is used for growing vegetables. Can all these be...
14 December 2023 7,049 1 View
I need a hard copy of the field guide for Odonate of Maharashtra, India.
05 December 2023 8,057 2 View
Odonates tend to glide, fly, hover, and move frequently. During transect surveys, it is possible to count one individual more than once if it flies back and forth. Is there any method (statistical...
30 October 2023 6,829 4 View
Inputs-Laser power Layer thickness Scan speed Hatch spacing Output- Relative density, UTS, Hardness, Yield strength, Surface roughness , % Elongation
21 October 2023 4,770 1 View
And please explain when we observe quantum confinement going below 10nm.
27 June 2023 9,884 0 View
So we have to do process the samples for chip seq and these are the cells of PANC - 1. My colleague fixated these cells 2 months back and we were practicing for running the samples. So we...
25 May 2023 3,509 5 View
In MEE section , How to calculate Vapour load ( gas to liquid )
19 March 2023 622 0 View
isolation of rhizobacteria from turmeric rhizomes but there is the contamination of fungus.
31 January 2023 7,492 2 View
working on the isolation of phosphate solubilizer by the use of Pikovaskayas media but after some days it was turned black although it was tightly packed what should i do
25 November 2022 9,398 2 View
I want to remove Urea from my protein (6kDa) sample. I did use : 1. Step down the Urea gradient (dialysis) in order to remove urea and refold my protein, but my protein still contains urea in the...
06 August 2024 1,350 4 View
kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
I am trying to adapt adherent CHO-K1 cells in F12K+10%FBS to suspension culture in only F12K, but in vain. I have followed Mirus bio as well as Thermo protocols, please suggest any alternative....
31 July 2024 3,756 0 View
I am conducting my research regarding blood coagulation in patients with CKD. I observed a reduction in post hemodialysis aPTT and PT values compared to pre hemodialysis values. But most of...
30 July 2024 3,467 0 View
We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?
29 July 2024 8,402 4 View
What should be the zeta potential of bovine milk exosomes
25 July 2024 7,376 5 View
hi, I am facing difficulties in the développement of an ELISA sandwich. I have several experiences in developing ELISA but that’s the first time I can’t find any solution. My test is currently as...
16 July 2024 9,044 10 View
In many experiments where HK-2 cells were stimulated with TGF-β at 10 ng/mL for 48 hours, I observed that even in the control group, the baseline expression level of fibrosis proteins like FN1 and...
15 July 2024 1,929 2 View
Hello everyone. I am working on my LV concentration porject, I use 500Kda hollowfiber to do it. The HCP and BSA removal rate is nearly 80%, but last time, I failed to run it. The HCP hasn't been...
11 July 2024 5,380 0 View