Is there any miRNA fluorescent labeling kit (or methods) that does not affect (add) the length of the miRNA?

I want to label my target miRNA and see the miRNA trafficking. But I don't want to use any plasmid or radioactive label. I need the fluorescent label that will not affect the length of my...

06 December 2020 8,471 3 View

Can too much of Tris-Cl buffer inhibit PCR?

I accidentally resuspended my stock primers (100 uM) in tris-Cl (pH 8.0) 1 M instead of 10 mM. I do PCR with 20 uM dilution from the stock. Will the high concentration of Tris-Cl inhibit PCR? I...

12 July 2020 2,971 10 View

Does hot KOH react with zirconia?

Hot KOH destroy many materials like alumina or glass.. So, can I use zirconia(ZrO2) boat for KOH activation of carbon nanoparticle experiments?

09 April 2020 800 1 View

Paired t-test or Independent samples t-test?

Hi, I am working on the home advantage. And examining the difference of a player's performance between playing at home and away. However, the sample size is different because the player played...

24 February 2020 7,053 3 View

Is it possible to wash PPFE slides that have been put through fluorescent reaction and stain them again?

I have some 3 year old PPFE slides of human brain that have been reacted with primary antibodies then reacted with Alexa Fluor secondary antibodies. The DAPI on the slides is still visible but the...

02 May 2019 7,390 2 View

What type of statistical tests on SPSS should I use for my study?

Hi I am carrying out a study investigating the association between age and password strategies among online users. I have collected my data, and inputted it on SPSS, however, I am not sure which...

10 December 2018 1,002 7 View

Can docking be used to predict a peptide's target receptor?

Let's say I have an array of receptors. Given a peptide, can protein-peptide docking be used in predicting the peptide's target among the receptors? If so, how? If not, why? Can you recommend me...

24 July 2018 3,569 4 View

Fc block not working for Flow Cytometry?

Good day all! I would greatly appreciate help with the following issue: Our lab is trying to characterize mouse lymphocytes for Tbet, EOMES, and PD-1. When I ran the samples the first time, the...

28 March 2018 1,225 3 View

In general speaking, how long can I keep antibody-labeled sections in wash buffer after the staining of secondary fluorescent antibody?

If the mounting is delayed for a day or two, would the specific binding being greatly reduced?

05 December 2017 4,103 3 View

Call for Papers: Australian Gender Economics Workshop (AGEW), Feb 2018, Perth Australia ?

15 October 2017 9,870 4 View

How do I determine the degree of polymerization of PEG 400 based on NMR integration?

Hello, I am working on the photocatalytic degradation of PEG 400. I would like to know if it's possible to determine the degree on polymerization based on NMR integration, or by the MW of the...

02 March 2021 6,186 3 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

Does the circuit modeling for planar monopole antennas using Foster canonical forms give good approximation?

Hi researchers, I am searching for some accurate way to do the circuit modeling of planar CPW-fed monopole antennas. I have gone through the literature where mostly circuit modeling is done using...

28 February 2021 7,016 1 View

How to include original NMR data in a manuscript?

hello. i am junior researcher. I received a comment from a reviewer that he could not find NMR data in my submitted paper. I had included the NMR graph with explanation and formula to calculate...

27 February 2021 734 4 View

How to Study a proton NMR of Graphene prepared by Electrochemical Exfoliation technique?

I performed a Proton NMR of graphene powder prepared by electrochemical exfoliation technique. It shows some peaks. But I can't understand it.

21 February 2021 8,947 3 View

Is There are any idea to achieve H NMR signals for swollen compound ??

the samples are not completely soluble - just swelled the solvents that's mean the sample may be partially soluble any suggestion, please

16 February 2021 8,801 3 View

How to detect uv vis spectrum of nanoparticle under 300nm?

I try to make the indium nanoparticle and check the uv vis spectrum. I heard that In nanoparticle has surface plasmon resonance peak at 200~300nm. But most of solvent has absorption peak under...

14 February 2021 6,950 15 View

How to derive equations for a resonant buck converter with ZVS?

I am not understanding how each author is deriving the equations and the working principle. Individual authors are giving their own working principle explanation for different circuit topology....

12 February 2021 496 1 View

How to increase coupling range (distance between reader and sensor coils) in LC resonance circuit?

Hello great researchers, I am working on a wireless pressure monitoring project where sensing part is actually an LC resonance sensor and I am using a planar coil as a reader to get wireless...

11 February 2021 4,148 5 View