Why does the BiFC（bimolecular fluorescence complementation）technique seem mostly used in Plant Research field but not animal cells?

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When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Can I use a protein-devoid condition as my negative control in ELISA?

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23 February 2021 7,429 1 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

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17 February 2021 4,844 4 View

What is the best temperature to coat an ELISA plate?

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16 February 2021 2,377 7 View

SPSS - Merging files by ID numbers that contain letters?

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15 February 2021 5,833 3 View

How would I go about getting an expert review on the interview questions for the dissertation?

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08 February 2021 346 7 View

How can I quantify the color of a solution?

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ACE2 Blotted as a double band?

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SARS-CoV-2 Spike Protein Western Bands?

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How does DMSO percentage affect ELISA?

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Why might my GFP tag be dissociating from my plasmid after transfection?

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01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

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What is the most suitable server for predicting protein structures in bacteria?

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28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

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28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

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28 February 2021 7,370 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

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25 February 2021 9,761 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

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24 February 2021 6,310 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

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24 February 2021 5,749 3 View

Can I use a protein-devoid condition as my negative control in ELISA?

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Is there a way to extract total RNA and protein from cell culture at the same time, without denaturing protein?

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22 February 2021 5,341 5 View

Sabine Strehl

I am not sure why BIFC is mostly used in plants but it does work in mammalian/human cells; see e.g.,

Article Study of the Bcl-2 Interactome by BiFC Reveals Differences i...

Article PDGFR dimer-specific activation, trafficking and downstream ...

Article Functional Heterogeneity of PAX5 Chimeras Reveals Insight fo...

Zabih Mir Hassani

Another example here:

https://academic.oup.com/nar/article/48/22/12900/6007657?login=false

I agree that it’s not used widely, but its applicable to any context!