David Genisys

4 Questions 4 Answers 0 Followers

Questions related from David Genisys

How to Calculate the Fold Change and P-value of absent proteins in IgG group of IP-MS data to plot a volcano plot?

Hello. We understand that a volcano plot is a graphical representation of differential values (proteins or genes), and it requires two parameters: fold change and p-value. However, for IP-MS...

26 November 2023 9,642 3 View

Why does the BiFC（bimolecular fluorescence complementation）technique seem mostly used in Plant Research field but not animal cells?

BiFC（bimolecular fluorescence complementation） is a useful method to study protein interaction. But what makes me surprised is that the usage is enriched in plant research field. I have no...

04 April 2023 2,639 2 View

Will TMT reacted with carbamidomethyled cystein since blocked Cystein contains a primary amino group gained from iodoacetamide?

I would like to ask a question. TMT labeled peptides by reacting with amino groups (primary amine groups), but the sulfhydryl-acetamide structure (carbamidomethyl of Cystein) formed after IAM...

01 April 2023 496 2 View

Why adherent cancer cells still have contact inhibition?

It is said that Cancer Cells have no contact inhibition and they can grow into multi layers. BUT，when we culture the HeLa cell, which is a well-known cancer cell line, in the plate, the HeLa cells...

30 March 2023 1,091 1 View