Can rats orally consume ketamine which is added in their drinking water?

Hello all, Has anyone tried to orally administer ketamine to mice or rats inside drinking water, wihout addition of any sugar? Do the animals really avoid drinking water with ketamine? I tried to...

01 September 2020 481 3 View

How to prepare miRNA-Seq library prep pipeline?

Hey everyone, I am trying to do a miRNA-Seq experiment, but as you all know, before doing so I need to prepare a library and when reading up on it and asking people from around the field I still...

28 July 2018 4,336 5 View

Is it possible to extract and purify miRNAs from frozen whole blood samples?

Hello everyone, I have been trying to find a reliable and reproducible method for extracting miRNAs from whole blood. I am aware there are PAXgene tubes, however the blood samples are collected...

25 April 2018 6,706 3 View

What are the applications of NGS systems that are useful for miRNA-Seq?

Hello everyone, For my project I need to do a comprehensive miRNA-Seq experiment and at the moment our collaborators only have a MiSeq System which as far as I know is a targeted sequencing...

26 October 2017 3,051 4 View

Affordable and Reliable RNA Sequencing Core Facility?

Hello everyone, For my research I need to do some RNA-Seq experiments in order to generate a miRNA profile from scratch from blood serum samples. However, we have a specific budget for this...

23 October 2017 585 3 View

Quantile Normalization and Data Processing

Hello everybody, I am fairly new to microarray expression analyses, therefore I am trying to reproduce data from raw data provided from a different research experiment. In the paper it...

10 August 2017 6,683 3 View

Why are my Ct values are vastly different from each other in qPCR? Also any tips on how to generate the graphs related to qPCR manually?

Hey everybody, I did a qPCR and gained results, however in some of my samples, one of my triplicates has a much higher Ct value than the others. This is quite frequent but only just one sample is...

15 August 2016 2,032 4 View

How to do cDNA synthesis without a kit?

Hello everyone, I have been having issues with the cDNA synthesis that I have been doing recently and wanted to check if there is anything that I can do to improve it as my results have not been...

03 August 2016 3,376 5 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Does any one can help me choose a reasonable basis set for intermediate metal ions interactions with guanine (i.e Fe2+, Mn2+, Cu2+ and exc.)?

Hello every body Does any one has any idea to help me choose a reasonable basis set for intermediate metal ions (i.e Fe2+, Mn2+, Cu2+ and exc.) interactions with guanine or other DNA organic...

01 March 2021 6,187 2 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

RNA degraded after good nanodrop readings?

23 February 2021 8,850 2 View