Ege Dedeoğlu

11 Questions 7 Answers 0 Followers

Questions related from Ege Dedeoğlu

Problems with one-step PCR?

Hello everyone, We are trying to set up a one-step PCR for COVID19. We are trying to implement the Midnight 1200bp, by using a one-step RT-PCR protocol instead of the recommended two-step...

05 July 2021 936 1 View

What are the weird peaks in my miRNA-Seq Library Prep?

Hey everyone, I am trying to do a miRNA-Seq experiment. I have prepared the libraries and before sequencing, I did QC measurements on the Agilent Bioanalyzer 2100, HS DNA Kit. I have some weird...

13 April 2021 8,154 1 View

Why does Inosine read as Guanine during translation, when Inosine can pair with both Adenine and Uracil?

Hey everyone, I am aware that Inosine preferentially binds with Cytosine, but in tRNAs inosine at the 34th postition acts as a wobble base and can pair with adenine and uracil also. I was...

25 January 2019 1,763 0 View

How to prepare miRNA-Seq library prep pipeline?

Hey everyone, I am trying to do a miRNA-Seq experiment, but as you all know, before doing so I need to prepare a library and when reading up on it and asking people from around the field I still...

29 July 2018 4,416 5 View

Is it possible to extract and purify miRNAs from frozen whole blood samples?

Hello everyone, I have been trying to find a reliable and reproducible method for extracting miRNAs from whole blood. I am aware there are PAXgene tubes, however the blood samples are collected...

26 April 2018 6,794 3 View

What is the significance of the endometrial profile during ovarian stimulation at the proliferative phase?

Hello everyone, I was researching the effects of ovarian stimulation regimens, mainly GnRH antagonists & agonist protocols on the endometrium. From what I read I understand that the main...

04 November 2017 2,189 4 View

What are the applications of NGS systems that are useful for miRNA-Seq?

Hello everyone, For my project I need to do a comprehensive miRNA-Seq experiment and at the moment our collaborators only have a MiSeq System which as far as I know is a targeted sequencing...

27 October 2017 3,143 4 View

Affordable and Reliable RNA Sequencing Core Facility?

Hello everyone, For my research I need to do some RNA-Seq experiments in order to generate a miRNA profile from scratch from blood serum samples. However, we have a specific budget for this...

24 October 2017 672 3 View

Quantile Normalization and Data Processing

Hello everybody, I am fairly new to microarray expression analyses, therefore I am trying to reproduce data from raw data provided from a different research experiment. In the paper it...

11 August 2017 6,759 3 View

Why are my Ct values are vastly different from each other in qPCR? Also any tips on how to generate the graphs related to qPCR manually?

Hey everybody, I did a qPCR and gained results, however in some of my samples, one of my triplicates has a much higher Ct value than the others. This is quite frequent but only just one sample is...

16 August 2016 2,116 4 View

How to do cDNA synthesis without a kit?

Hello everyone, I have been having issues with the cDNA synthesis that I have been doing recently and wanted to check if there is anything that I can do to improve it as my results have not been...

04 August 2016 3,449 5 View