How can I calculate the value of a concentration of enzyme from optical density?

optical density was measured at 270 nm for enzyme assay after sample collection time interval of 15 sec

10 November 2017 884 5 View

What would be the ideal conditions of HPLC for organic acid detection? Which mobile phase and column is suitable, as organic acids are hydrophilic?

Analytical method for organic acid detection using HPLC

10 November 2017 3,371 3 View

Which bioinformatic tool is feasible to explain metagenomic data of soil microbes?

Hi, I'm working on soil microbe diversity and after extracting whole microbe DNA from particular soil, I'v metagenomic data by pyro sequencing which tells me the diversity of microbes in soil. I...

10 November 2017 5,735 11 View

How Can I determine functional gene community for diversity studies?

Hi,I'm working on degradation studies. The sample I have, is a consortia of unknown microorganisms. I want to study diversity of microorganisms on gene basis particularly functional gene...

01 February 2017 8,964 4 View

How can I calculate production of carbonic anhydrase?

10 November 2016 1,553 0 View

Does anyone has Experience in elaborating GCMS results?

06 July 2016 3,863 2 View

How can I check the reductase activity in a Liquid Solution?

Hi, I'M working on the soil Enzymes. I need to to check the total reductase enzyme production and its activity produced by certain enzymes especially when the inoculum is converted to liquid...

01 February 2016 7,364 2 View

What is the best extraction solvent for textile dyes?

31 December 2015 1,193 3 View

Does Vitamins produce energy?

Does vitamins produce specific amount of energy or they are only precursors for the metabolites?

07 August 2015 8,897 4 View

What kind of media or material can be utilized for minimizing foaming in laboratory anaerobic membrane bioreactor?

01 January 1970 3,118 0 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

In methylene blue test Why is UV absorbance reading very low as soon adsorbent is added and then increasing before reaching equilibrium?

Hello, I'm working on removal of methylene blue. 01. in the dark test very first reading is taken 15 minutes after adding adsorbent to the dye solution and that UV absorbance reading is very...

19 February 2021 472 5 View

Has anyone performed a colorimetric RT-LAMP assay using Lucigens LavaLAMP kit?

Hi there, my lab recently opted to use LavaLAMP from Lucigen instead of the Warmstart kit from NEB. However, Lucigen has yet to create a colormetric kit for RT-LAMP, and adding phenol-Red (pH...

17 February 2021 5,399 1 View

Why stop the reaction in ice for the fluorescent probe FDA (fluorescein diacetate)?

Hi, In literature I noticed that who stains haemocytes with FDA for 30 min then stop the reaction in ice while this does not apply for 15 min incubation time (or maybe authors did not write this...

08 February 2021 3,366 3 View

What is the possible cause of MSCs low growing?

Hello Everyone, We are inexperienced in culture of MSCs trying to grow a sample from BM of a mare. It is the second time that after preparation of new medium (DMEM-F12 with high glucose - 10% Hy...

07 February 2021 9,989 7 View

Why do cells die after adding MTT dye?

I was doing MTT assay, and in the last day before adding the MTT; the cells looked good (or so I think), but after aspiration of the drug and addition of MTT dye I noticed that most cells seemed...

07 February 2021 3,846 4 View

How to measure the concentration of a labeled protein with no tryptophan or tyrosines?

Hey I'm trying to measure the concentration of a protein I've purified and then labeled with Atto 647 flourescent dye. The protein has no tryptophans or tyrosines (although does have...

04 February 2021 4,482 4 View

Why are my western blot bands so distorted?

Hi guys I'm out of ideas here please help! I've never had consistent problem like this doing westerns before and I can't figure it out. The condition used: - First 4 lanes have 50ug protein in...

04 February 2021 2,297 3 View

How to remove unreacted florescent dye from labeled protein?

Hi I have a protein labeled with rhodamine B and after reaction i used Zeba spin column for dye removal. my problem is that lot of unreacted dye remained which interfered with my florescent...

02 February 2021 644 2 View