How can I calculate the value of a concentration of enzyme from optical density?

optical density was measured at 270 nm for enzyme assay after sample collection time interval of 15 sec

10 November 2017 884 5 View

What would be the ideal conditions of HPLC for organic acid detection? Which mobile phase and column is suitable, as organic acids are hydrophilic?

Analytical method for organic acid detection using HPLC

10 November 2017 3,371 3 View

Which bioinformatic tool is feasible to explain metagenomic data of soil microbes?

Hi, I'm working on soil microbe diversity and after extracting whole microbe DNA from particular soil, I'v metagenomic data by pyro sequencing which tells me the diversity of microbes in soil. I...

10 November 2017 5,735 11 View

How Can I determine functional gene community for diversity studies?

Hi,I'm working on degradation studies. The sample I have, is a consortia of unknown microorganisms. I want to study diversity of microorganisms on gene basis particularly functional gene...

01 February 2017 8,964 4 View

Does anyone has Experience in elaborating GCMS results?

06 July 2016 3,863 2 View

How can I check the reductase activity in a Liquid Solution?

Hi, I'M working on the soil Enzymes. I need to to check the total reductase enzyme production and its activity produced by certain enzymes especially when the inoculum is converted to liquid...

01 February 2016 7,364 2 View

What is the best extraction solvent for textile dyes?

31 December 2015 1,193 3 View

Does Vitamins produce energy?

Does vitamins produce specific amount of energy or they are only precursors for the metabolites?

07 August 2015 8,897 4 View

Why do we use MSM for the treatment of dyes rather than broth or other media?

Why do we use MSM for the treatment of dyes rather than broth or other media?

05 June 2015 8,806 2 View

What kind of media or material can be utilized for minimizing foaming in laboratory anaerobic membrane bioreactor?

01 January 1970 3,118 0 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

How can I integrate a routing protocol in a tiny device (e.g. Raspberry Pi)?

Hi Community, I'm facing the issue of integration/compiling a new routing protocol in a WSN simulator. The final goal is to successfully add, configure this routing protocol in hardware devices...

01 March 2021 9,332 6 View

How to calculate the fraction metabolized of a drug by an enzyme using clearance(ml/min) values of the metabolite formed?

I am trying to find the fraction metabolized of a drug by CYP and UGT enzymes. I am unable to get the values of fraction metabolized (Fm) directly from the literature. Can we calculate the Fm of a...

21 February 2021 5,379 2 View

How can I add a new WSN routing protocol in NS2 or NS3?

I'm trying to find in literature a way to help me adding a modified Yen's algorithm in ns2 or its variance ns3 or another simulator. Have you some tips which can be helpful, I'm opening in the...

19 February 2021 7,981 3 View

How long does it take to design and validate transition state analogue inhibitors?

Hi, I'm asking from the point of view of designing a research plan: How long (approximately) can it take to determine the transition state structure of an enzyme substrate using kinetic isotope...

14 February 2021 2,610 1 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View

No title

Hello! I want to determine the activity of my in house Reverse Transcriptase enzyme. For this, I have used Thermo's Enzcheck Reverse Transcriptase assay kit. I used commercial Thermo RT enzyme to...

08 February 2021 7,285 1 View

How define one baker unit(BU) is the amount of hydrogen peroxide catalase ?

07 February 2021 2,006 1 View

Does DNA A-tailing survive after agarose gel electrophoresis and purification?

Hi everyone, I need to clone in pGEM-T vector the result of amplifying a metagenomic library by PCR with a high fidelity polymerase but I only want to clone the fragments of an specific size...

04 February 2021 6,051 8 View

What are some common oxidants used to oxidize iron sulfur clusters in proteins for techniques like EPR?

I have used potassium ferricyanide (which is likely the most common) extensively in the past, but I am curious to know if there are other oxidants that work well. I need to generate the oxidized...

29 January 2021 9,803 4 View