How can I calculate the value of a concentration of enzyme from optical density?

optical density was measured at 270 nm for enzyme assay after sample collection time interval of 15 sec

10 November 2017 884 5 View

What would be the ideal conditions of HPLC for organic acid detection? Which mobile phase and column is suitable, as organic acids are hydrophilic?

Analytical method for organic acid detection using HPLC

10 November 2017 3,371 3 View

Which bioinformatic tool is feasible to explain metagenomic data of soil microbes?

Hi, I'm working on soil microbe diversity and after extracting whole microbe DNA from particular soil, I'v metagenomic data by pyro sequencing which tells me the diversity of microbes in soil. I...

10 November 2017 5,735 11 View

How Can I determine functional gene community for diversity studies?

Hi,I'm working on degradation studies. The sample I have, is a consortia of unknown microorganisms. I want to study diversity of microorganisms on gene basis particularly functional gene...

01 February 2017 8,964 4 View

How can I calculate production of carbonic anhydrase?

10 November 2016 1,553 0 View

How can I check the reductase activity in a Liquid Solution?

Hi, I'M working on the soil Enzymes. I need to to check the total reductase enzyme production and its activity produced by certain enzymes especially when the inoculum is converted to liquid...

01 February 2016 7,364 2 View

What is the best extraction solvent for textile dyes?

31 December 2015 1,193 3 View

Does Vitamins produce energy?

Does vitamins produce specific amount of energy or they are only precursors for the metabolites?

07 August 2015 8,897 4 View

Why do we use MSM for the treatment of dyes rather than broth or other media?

Why do we use MSM for the treatment of dyes rather than broth or other media?

05 June 2015 8,806 2 View

What kind of media or material can be utilized for minimizing foaming in laboratory anaerobic membrane bioreactor?

01 January 1970 3,118 0 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

Necessary tube lenght to cool Natural gas in a dry-ice cooling bath?

I have a tank which contains natural gas at 250 Bar and 293 Kelvin. I want to cool the gas in a dry-ice cooling bath to about 195 Kelvin. The gas will flow in a copper-tube through the bath with a...

27 February 2021 8,128 1 View

Exit velocity of natural gas from pressurized tank?

Hi Guys. I'm a mechanical engineer and have a bit trouble, would be very glad if you could help me with my questions. I have a pressurized tank which contains Natural Gas at 200 Bar and 293...

26 February 2021 6,253 3 View