How can I calculate the value of a concentration of enzyme from optical density?

optical density was measured at 270 nm for enzyme assay after sample collection time interval of 15 sec

10 November 2017 884 5 View

What would be the ideal conditions of HPLC for organic acid detection? Which mobile phase and column is suitable, as organic acids are hydrophilic?

Analytical method for organic acid detection using HPLC

10 November 2017 3,371 3 View

Which bioinformatic tool is feasible to explain metagenomic data of soil microbes?

Hi, I'm working on soil microbe diversity and after extracting whole microbe DNA from particular soil, I'v metagenomic data by pyro sequencing which tells me the diversity of microbes in soil. I...

10 November 2017 5,735 11 View

How can I calculate production of carbonic anhydrase?

10 November 2016 1,553 0 View

Does anyone has Experience in elaborating GCMS results?

06 July 2016 3,863 2 View

How can I check the reductase activity in a Liquid Solution?

Hi, I'M working on the soil Enzymes. I need to to check the total reductase enzyme production and its activity produced by certain enzymes especially when the inoculum is converted to liquid...

01 February 2016 7,364 2 View

What is the best extraction solvent for textile dyes?

31 December 2015 1,193 3 View

Does Vitamins produce energy?

Does vitamins produce specific amount of energy or they are only precursors for the metabolites?

07 August 2015 8,897 4 View

Why do we use MSM for the treatment of dyes rather than broth or other media?

Why do we use MSM for the treatment of dyes rather than broth or other media?

05 June 2015 8,806 2 View

What kind of media or material can be utilized for minimizing foaming in laboratory anaerobic membrane bioreactor?

01 January 1970 3,118 0 View

How does the gut microbiome affect the fibrotic process within the liver?

and Can the gut microbe be used for the treatment or diagnosis of liver fibrosis?

02 March 2021 557 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

The Shannon's index is inversely proportional to Simpson's index (1-D)?

I'm having a problem with my bloxplot with Shannon's and Simpson's indexs. The value of Shannon for my Community 1 indicating higher diversity (in relation of the Community 2), but have lower...

26 February 2021 3,033 9 View

How to indicate the degree of litter decomposed by microbes?

Any technique could detect the degree of litter decomposed by microbes? Or any indexes could do this job? I want to split the contribution of microbes during decomposition, so any suggestions...

23 February 2021 1,362 5 View

Can I get information about research laboratories that are currently having in culture, internationally/locally standardised microorganisms?

Microorganisms of interest include; E.coli; Salmonella typhimurium; Pseudomonas aeroginosa; Streptoccocus pneumoniae; Staphyloccocus aureus and Candida albicans. Locations closer to Port...

18 February 2021 6,500 3 View

Any recommendations for methods to analyze elephant space-use (using GPS) and correlating this with changes in NDVI?

For my Masters I am looking into elephant space-use and its effect on vegetation change. I have elephant GPS collar data and was going to use Google Earth Engine to obtain NDVI values. I would...

16 February 2021 7,097 3 View

How to consider effects of rapid warm adaptation in modeling of ecological impacts of warming?

I would highly appreciate it if my fellow ecologists (biologists) provide their opinion on the thoughts below [esp., shortly tell us which path may be more effective, if they know another way, if...

15 February 2021 3,642 6 View

Can one use just 250 micron sieve & still get a good number of mycorrhizal spores from the soil?

In a situation where one cannot get the appropriate & separate levels of sieve for mycorrhizal survey work, can just one level of sieve give good results & spore counts?

13 February 2021 3,760 2 View

How do microbes tell the presence of an inorganic minaral in the soil?

Due to their ubiquitous nature, there is a tendency of the presence of microbes in a given mineral(s). For instance, if microorganisms that love the of pH less than 6 in the pH value, is isolated...

03 February 2021 4,630 10 View

Is an Algal Microbial Fuel Cell a good research topic?

What do you think about studying the electricity production in an Algal Microbial Fuel Cell? Namely at the anode there is for example a solution with bacteria which degradate organic matter into...

27 January 2021 5,681 5 View