Why do we use different antibiotic resistance chosen in part vectors and destination vectors in golden gate assembly?

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Katie A S Burnette

As a "though experiment", diagram out what would happen if you only had 1 antibiotic resistance gene. You'll see the logic for why you need 2 genes.

Subham Basak

Hi Yukti Kataria

This as i understand, will make it easier for you to select the cells having complete assembly of the vector.

Hope it helps,

Subham.

Yukti Kataria

Thank you Subham Basak