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Questions related from Subham Basak
Also would like to know the ExpiCHO parameters like ellipticity/ circularity and max/min. diameter to count the cells via automated cell counters. Thanks for the help!
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I am doing HEK293 antibiotic selection after transfection, and everyday i find dead cells collected at the center of the well, although the plate is kept static in the incubator. Any plausible...
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Hi everyone, I want to increase the biomass of my E. coli culture for better plasmid yield. Use of different strains of cells and media or any other suggestions are welcome. Thanks!
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It is seen that most diagnostics kits are Taqman based, now as PCR is quite sensitive, can we settle ourselves with SYBR based kits in a resource-crunch set up?
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I have isolated RNA from 50ul serum and eluted the RNA in 30ul of DEPC- treated water. But when i am preparing 10ul of cDNA from 1ul of the RNA and using it for amplification of a 115bp...
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I would like to get learned insights into detection of COVID-19 using techniques beyond the routine RTqPCR from you all. Thanks for your thoughts!
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