Why do we use Amino Acids in culture medium?

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Why do i have basal expression after i put my bacterial bank in the freezer?

Hello I have done a cloning and after that i have stored my recombinant E.coli cells in the -80C and -20C as a cell banks. after i have used the cells for expression i saw a basal expression in...

10 November 2017 4,628 13 View

What should i do in order to calculate my purification purity?

Hello.I have purified my protein with Ni-NTA coulmn and i have run my purified fractions on SDS-PAGE but i don't know how to calculate the purity of may bands. how should i do this?Thanks in advance.

06 July 2017 3,184 8 View

Why does sodium phosphate buffer precipitates?

HelloI am trying to purify human growth hormone with His6 tag. thus i should make buffers with different imidazole concentration. i made my buffer solution at room temperature. but when i put...

01 February 2017 7,320 10 View

Is there any video tutorial for CLIQS gel analyzer?

Hello dears. CLIQS(stands for Core Laboratory Image Quantification Software) from totallab company is one of the gelanalyzer software like ImageJ but i want some tutorial to learn it well( any...

10 November 2016 6,009 0 View

How do I ensure that my sonication has done perfectly?

Hello How do I ensure that my sonication has been done perfectly and my cell pellets were lysed completely?Any test or procedure? Thanks in advance.

10 November 2016 3,611 4 View

Is it possible that a produced recombinant protein shows no activity?

Hello I want to work on production of a recombinant protein(recombinant human growth hormone) in a laboratory and the answer of this question is very important to me. Is it Possible that after...

09 October 2016 7,537 8 View

Problem with SDS-PAGE Running? protein's bands dont migrate fully?

Hello dears. I did run SDS-PAGE gel yesterday, after staining the gel and destaining the gel i saw that protein's bands did not migrate to the end of the gel and they have just migrated half of...

06 July 2016 3,855 7 View

How to plot HPLC Chromotogram without HPLC system?

Hello I want to plot my chrmatographic column's chromotoramme but i don't have access to HPLC system and i dont know how to get my protein peak therefore i should make my own chromotogramme...

06 July 2016 5,491 10 View

What is SDS-PAGE making principle?

Hello I don't understand the relation between reagents used in SDS-PAGE making. I mean there are many protocols that say how to make SDS-PAGE, for example 15%, but they don't mention how they...

31 December 2015 1,836 4 View

Which method is good for periplasmic lysis of Escherichia coli?

Hello which methods are good to disrupt the E.coli periplasmic space to extract protein and what is the mechanism of each method? Thanks in advance.

31 December 2015 3,364 6 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

How Social Media Affects Your Mental Health ?

How Social Media Affects Your Mental Health

04 August 2024 6,961 3 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View