Nii Sama

11 Questions 27 Answers 0 Followers

Questions related from Nii Sama

Why do i have basal expression after i put my bacterial bank in the freezer?

Hello I have done a cloning and after that i have stored my recombinant E.coli cells in the -80C and -20C as a cell banks. after i have used the cells for expression i saw a basal expression in...

11 November 2017 4,649 13 View

What should i do in order to calculate my purification purity?

Hello.I have purified my protein with Ni-NTA coulmn and i have run my purified fractions on SDS-PAGE but i don't know how to calculate the purity of may bands. how should i do this?Thanks in advance.

07 July 2017 3,194 8 View

Why does sodium phosphate buffer precipitates?

HelloI am trying to purify human growth hormone with His6 tag. thus i should make buffers with different imidazole concentration. i made my buffer solution at room temperature. but when i put...

02 February 2017 7,323 10 View

Is there any video tutorial for CLIQS gel analyzer?

Hello dears. CLIQS(stands for Core Laboratory Image Quantification Software) from totallab company is one of the gelanalyzer software like ImageJ but i want some tutorial to learn it well( any...

11 November 2016 6,012 0 View

How do I ensure that my sonication has done perfectly?

Hello How do I ensure that my sonication has been done perfectly and my cell pellets were lysed completely?Any test or procedure? Thanks in advance.

11 November 2016 3,614 4 View

Is it possible that a produced recombinant protein shows no activity?

Hello I want to work on production of a recombinant protein(recombinant human growth hormone) in a laboratory and the answer of this question is very important to me. Is it Possible that after...

10 October 2016 7,545 8 View

Problem with SDS-PAGE Running? protein's bands dont migrate fully?

Hello dears. I did run SDS-PAGE gel yesterday, after staining the gel and destaining the gel i saw that protein's bands did not migrate to the end of the gel and they have just migrated half of...

07 July 2016 3,858 7 View

How to plot HPLC Chromotogram without HPLC system?

Hello I want to plot my chrmatographic column's chromotoramme but i don't have access to HPLC system and i dont know how to get my protein peak therefore i should make my own chromotogramme...

07 July 2016 5,499 10 View

Why do we use Amino Acids in culture medium?

Hello Why do we use Amino Acids in culture medium, i mean what is the mechanism of used amino acids in molecular point of view? What is they role in cell mechanism? any publication? Thanks in...

02 February 2016 8,261 2 View

What is SDS-PAGE making principle?

Hello I don't understand the relation between reagents used in SDS-PAGE making. I mean there are many protocols that say how to make SDS-PAGE, for example 15%, but they don't mention how they...

01 January 2016 1,844 4 View

Which method is good for periplasmic lysis of Escherichia coli?

Hello which methods are good to disrupt the E.coli periplasmic space to extract protein and what is the mechanism of each method? Thanks in advance.

01 January 2016 3,371 6 View