Why do Ultrafiltered ( using 100kd mwco filter) Brucella abortus s19 omv show band only with silver staining, and not by Coomasie blue on SDS page?

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Martin Koethe

Maybe this is a question of concentration. Silver staining is usually more sensitive than Coomassie, so also lower concentrations can be detected with silver staining.

Ke-Wei Zhao

Khushal: Ultrafilter membrane of 100 kD is not an exact size, and often means to eliminate globular proteins of that size. It is possible that your s19 fraction got filtered through and only minor proteins found in silver stain.