Can a single dosage of Brucella abortus S19 OMV(15micro gram) provide adequate protection against challenge in mice ?

06 July 2016 3,519 1 View

Why do Ultrafiltered ( using 100kd mwco filter) Brucella abortus s19 omv show band only with silver staining, and not by Coomasie blue on SDS page?

Why do Ultrafiltered ( using 100kd mwco filter) Brucella abortus s19 omv show band only with silver staining, and not by Coomasie blue on SDS page?

31 December 2015 1,900 2 View

How does Brucella Abortus Strain 19 provide life long immunity in cattle in single vaccination, as it persist in mouse spleen for almost 4 months?

How does Brucella Abortus Strain 19 provide life long immunity in cattle in single vaccination, as it persist in mouse spleen for almost 4 months?

04 May 2015 4,461 2 View

How can I get high yield of outer membrane vesicles from brucella abortus s19 culture?

HOW can i get high yield of Outer membrane vesicles from Brucella abortus s19 culture ? From solid Brucella agar or Brucella broth in what amount ?

31 December 2014 9,824 1 View

Why does sodium lauryl sarkosinate only solubilize the inner membrane of bacteria ?

Why does sodium lauryl sarkosinate only solubilize the inner membrane of bacteria ?

31 December 2014 2,581 2 View

Why gram negative bacterial outer membrane do not get solubilised by Sodium lauryl sarkosinate ?

Please reply at molecular level.

31 December 2014 9,039 4 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

How to plot the probability paper of Gumbel distribution using R programing?

In R-studio, there are many commands of Gumbel package. Arguments are also different. I`m asking about the alpha parameter of the Copula which must be greater than 1. If this is the one used to...

25 February 2021 5,229 3 View

Is there an appropriate CD8 antibody for IHC-P for staining human breast cancer tissues?

Hello, We are planning on staining Formalin-fixed paraffin breast cancer tissue sections (FFPE) with CD8 Ab via immunofluorescence (Tissue IF). Based on our existing protocol and imaging...

24 February 2021 9,578 4 View

Alginate and silk fibroin hydrogels toxic to cells?

Hello, I asm currently working with alginate and silk fibroin hydrogels. I prepared a blend of alginate and aqueous silk fibroin (straight from dialysis) and resorted to ionic crosslinking to...

23 February 2021 8,249 2 View

Are there methods for automatically mapping floodplains, fans and terraces?

I want to map these river geoforms with as little subjectivity as possible. I have a high resolution DEM generated by drone data.

22 February 2021 7,800 3 View

Are there any databases for gene co-expression or expression pattern clustering?

I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...

22 February 2021 4,374 3 View

Do I need to introduce HFD to ob/ob mice to obtain a T2DM model?

I am planning to work on T2 diabetic animals and check the efficacy of probiotics. My initial plan is to fed B6 animals with HFD for a while. But now, I have the chance to work on ob/ob mice. My...

18 February 2021 6,896 3 View

What are the options for global scale hydrology DEM?

Hello, I am working on a global scale hydrology GIS project delineating global watersheds and doing some data analysis. I have in the past used hydro1K from the USGS that cover the entire world....

18 February 2021 5,109 5 View

Wat is the half-life of luciferase protein in mouse tissue when delivered using mRNA luciferase?

Hello, I I am validating a system to deliver mRNA using nanoparticles to a mouse model. I am initially using mRNA for firefly luciferase to be able to do live imaging in the mouse. After that, I...

17 February 2021 2,536 3 View