Can a single dosage of Brucella abortus S19 OMV(15micro gram) provide adequate protection against challenge in mice ?

07 July 2016 1 4K Report

Can a single dosage of Brucella abortus S19 OMV(15micro gram) provide adequate protection against challenge in mice ?Why booster require?

Amrou E Ewida

You need a booster dose with incomplete adjuvant in order to get a long lasting immune response in case of searching for a vaccine candidate. However, if your aim is to search for a diagnostic candidate , the first dose should be sufficient.

Badges
Science topic

Similar topics
Eutheria
Rodentia
Muridae
Murinae
Mice

More Khushal Solanki's questions See All

What is the protocol for Mouse cd4 and cd8 analysis through FACS from whole blood?

04 May 2016 8,500 1 View

Why do Ultrafiltered ( using 100kd mwco filter) Brucella abortus s19 omv show band only with silver staining, and not by Coomasie blue on SDS page?

31 December 2015 1,900 2 View

How does Brucella Abortus Strain 19 provide life long immunity in cattle in single vaccination, as it persist in mouse spleen for almost 4 months?

04 May 2015 4,461 2 View

How can I get high yield of outer membrane vesicles from brucella abortus s19 culture?

HOW can i get high yield of Outer membrane vesicles from Brucella abortus s19 culture ? From solid Brucella agar or Brucella broth in what amount ?

31 December 2014 9,824 1 View

Why does sodium lauryl sarkosinate only solubilize the inner membrane of bacteria ?

31 December 2014 2,581 2 View

Why gram negative bacterial outer membrane do not get solubilised by Sodium lauryl sarkosinate ?

Please reply at molecular level.

31 December 2014 9,039 4 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Is the sinovac vaccine safe and effective for people over the age of 60 ?

Dear respective researchers, I was asked by my institution to be vaccinated this week using the sinovac vaccine. However, from some of the information I got, this vaccine is not recommended for...

01 March 2021 7,781 6 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Is there any published or unpublished statistics on dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, US, UK or any other country?

Are there any published or unpublished statistics or papers on the dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, USA, UK, or any other country where vaccination has started at...

28 February 2021 4,237 1 View